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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

DOI :

10.3791/61938-v

7:14 min

May 17th, 2021

May 17th, 2021

3,883 Views

1Department of Stem Cell Biology, Research Institute, National Center for Global Health and Medicine

This protocol enables the maintenance of quiescent human hematopoietic stem cells in vitro by mimicking the bone marrow microenvironment using abundant fatty acids, cholesterol, lower concentrations of cytokines, and hypoxia.

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Quiescent Human Hematopoietic Stem Cells

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