Toronto Metropolitan University (Formerly Ryerson Univ.)

2 ARTICLES PUBLISHED IN JoVE

Chemistry

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Cheuk Y. Ho^*1, Christopher H. Choy^*1, Roberto J. Botelho¹

¹Department of Chemistry and Biology, Program in Molecular Science, Ryerson University

Phosphoinositides are signaling lipids whose relative abundance rapidly changes in response to various stimuli. This article describes a method to measure the abundance of phosphoinositides by metabolically labeling cells with ³H-myo-inositol, followed by extraction and deacylation. Extracted glycero-inositides are then separated by high-performance liquid chromatography and quantified by flow scintillation.

Medicine

Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model

Lee Chin^1,2, Elina Korpela³, Anthony Kim¹, Darren Yohan², Carolyn Niu⁴, Brian C. Wilson³, Stanley K. Liu^1,3

¹Department of Radiation Oncology, University of Toronto, ²Department of Physics, Ryerson University, ³Department of Medical Biophysics, University of Toronto, ⁴Ontario Cancer Institute / Campbell Family Institute for Cancer Research

We present a diffuse optical spectroscopic (DOS) approach that provides quantitative optical biomarkers of skin response to radiation. We describe DOS instrumentation design, optical parameters extraction algorithms and the animal handling procedures required to yield representative data from a pre-clinical mouse model of radiation induced erythema.