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Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

10.3K Views

10:52 min

January 6th, 2016

DOI :

10.3791/53529-v

January 6th, 2016


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Keywords Radiolabeling

Chapters in this video

0:05

Title

0:58

Yeast Cell Culture and Radiolabeling of Phosphoinositides

2:58

Deacylation and Extraction of the Radiolabeled Phosphoinositides

5:14

Separation of Tritium-labeled Glycero-inositides by High Pressure Liquid Chromatography (HPLC)

7:15

Data Analysis

8:55

Results: HPLC Analysis of Radiolabeled Phosphoinositides

9:55

Conclusion

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