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Tri-service general hospital

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment
Gang-Yi Fan 1, Juin-Hong Cherng 2,3, Shu-Jen Chang 4, Raju Poongodi 2, Chih-Hsin Wang 5, Yi-Wen Wang 2, Yao-Horng Wang 6, Cheng-Che Liu 7, En Meng 8
1Graduate Institute of Medical Sciences, National Defense Medical Center, 2Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, 3Department of Gerontological Health Care, National Taipei University of Nursing and Health Sciences, 4Division of Rheumatology/Immunology/Allergy, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, 5Department of Plastic and Reconstructive Surgery, Tri-Service General Hospital, 6Department of Nursing, Yuanpei University, 7Department of Physiology and Biophysics; Graduate Institute of Physiology, National Defense Medical Center, 8Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center

To closely mimic the mode of burn injuries requires the interplay between clinical observation and studies in animal models. In this study, a swine model of severe burn injury was established to assess an experimental dressing in physiological and pathophysiological settings.

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Medicine

Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium
Chun Hsiung *1,2, Yu-Ting Liu *1,2, Chen-Ying Su 3, Chung-Chuan Hsiung 4,5, Kuo-Hsuan Hung 2,6,7, Lung-Kun Yeh 2,6
1Department of Education, Chang Gung Memorial Hospital Linkou, 2School of Medicine, Chang Gung University, 3Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 4Cell Therapy Center, Tri-Service General Hospital, 5Department of General Surgery, Bo-Ai Clinic, 6Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, 7Institute of Clinical Medicine, National Yang Ming Chiao Tung University

This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.

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