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Tri-service general hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

Gang-Yi Fan¹, Juin-Hong Cherng^2,3, Shu-Jen Chang⁴, Raju Poongodi², Chih-Hsin Wang⁵, Yi-Wen Wang², Yao-Horng Wang⁶, Cheng-Che Liu⁷, En Meng⁸

¹Graduate Institute of Medical Sciences, National Defense Medical Center, ²Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, ³Department of Gerontological Health Care, National Taipei University of Nursing and Health Sciences, ⁴Division of Rheumatology/Immunology/Allergy, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, ⁵Department of Plastic and Reconstructive Surgery, Tri-Service General Hospital, ⁶Department of Nursing, Yuanpei University, ⁷Department of Physiology and Biophysics; Graduate Institute of Physiology, National Defense Medical Center, ⁸Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center

To closely mimic the mode of burn injuries requires the interplay between clinical observation and studies in animal models. In this study, a swine model of severe burn injury was established to assess an experimental dressing in physiological and pathophysiological settings.

Medicine

Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium

Chun Hsiung^*1,2, Yu-Ting Liu^*1,2, Chen-Ying Su³, Chung-Chuan Hsiung^4,5, Kuo-Hsuan Hung^2,6,7, Lung-Kun Yeh^2,6

¹Department of Education, Chang Gung Memorial Hospital Linkou, ²School of Medicine, Chang Gung University, ³Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, ⁴Cell Therapy Center, Tri-Service General Hospital, ⁵Department of General Surgery, Bo-Ai Clinic, ⁶Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, ⁷Institute of Clinical Medicine, National Yang Ming Chiao Tung University

This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.