Hamad Medical Corporation
2 ARTICLES PUBLISHED IN JoVE
Biology
Evaluation of the Impact of a New Cooling Cell Processor System on Islet Cell Isolation Facility
Naziha Menasria 1,2, Marten Engelse 3, Julien Thévenet 2, Weam El Roz 1, Ayan Guled 1, Abdul-Badi Abou-Samra 1, François Pattou 2, Julie Kerr -Conte 2
1Qatar Metabolic Institute, Hamad Medical Corporation, 2Univ-Lille, Inserm, CHU Lille, Institute Pasteur Lille, U1190 - EGID F-59000 Lille, 3Department of Nephrology, Leiden University Medical Center (LUMC)
The protocol here describes a custom cooling cell processor system that is compatible with a good manufacturing practices (GMP) cleanroom to improve cell viability post-processing.
Bioengineering
Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
Sukumaran Anil 1,2, Nebu G. Thomas 2, Elna P. Chalisserry 2, Yogesh B. Dalvi 2, Ramya Ramadoss 3, Sajith Vellappally 4
1Department of Dentistry, Oral Health Institute, Hamad Medical Corporation, 2Pushpagiri Institute of Medical Sciences and Research Centre, 3Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, 4College of Applied Medical Sciences, King Saud University
This protocol emphasizes the extraction, culture, and preservation of multipotent stem cells from dental pulp through enzymatic digestion. Additionally, it demonstrates their potential to differentiate into osteoblasts, adipocytes, and chondrocytes, highlighting the importance of precision and consistency in the process.
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