Accedi

Hamad Medical Corporation

2 ARTICLES PUBLISHED IN JoVE

Biology

Evaluation of the Impact of a New Cooling Cell Processor System on Islet Cell Isolation Facility

Naziha Menasria^1,2, Marten Engelse³, Julien Thévenet², Weam El Roz¹, Ayan Guled¹, Abdul-Badi Abou-Samra¹, François Pattou², Julie Kerr -Conte²

¹Qatar Metabolic Institute, Hamad Medical Corporation, ²Univ-Lille, Inserm, CHU Lille, Institute Pasteur Lille, U1190 - EGID F-59000 Lille, ³Department of Nephrology, Leiden University Medical Center (LUMC)

The protocol here describes a custom cooling cell processor system that is compatible with a good manufacturing practices (GMP) cleanroom to improve cell viability post-processing.

Bioengineering

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Sukumaran Anil^1,2, Nebu G. Thomas², Elna P. Chalisserry², Yogesh B. Dalvi², Ramya Ramadoss³, Sajith Vellappally⁴

¹Department of Dentistry, Oral Health Institute, Hamad Medical Corporation, ²Pushpagiri Institute of Medical Sciences and Research Centre, ³Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, ⁴College of Applied Medical Sciences, King Saud University

This protocol emphasizes the extraction, culture, and preservation of multipotent stem cells from dental pulp through enzymatic digestion. Additionally, it demonstrates their potential to differentiate into osteoblasts, adipocytes, and chondrocytes, highlighting the importance of precision and consistency in the process.