R Adams Cowley Shock Trauma Center

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Novel Microsurgical Model for Heterotopic, En Bloc Chest Wall, Thymus, and Heart Transplantation in Mice

Byoungchol Oh^*1, Georg J. Furtmüller^*1, Michael Sosin^*1, Madeline L. Fryer¹, Lawrence J. Gottlieb^2,3, Michael R. Christy⁴, Gerald Brandacher^1,5,6, Amir H. Dorafshar^1,5,6

¹Johns Hopkins University School of Medicine, ²Burn and Complex Wound Center, ³Section of Plastic and Reconstructive Surgery, University of Chicago Medical Center, ⁴Division of Plastic, Reconstructive, and Maxillofacial Surgery, R Adams Cowley Shock Trauma Center, ⁵Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine, ⁶Vascularized Composite Allotransplantation (VCA) Lab, Johns Hopkins University School of Medicine

To study combined solid organ and vascularized composite allotransplantation, we describe a novel heterotopic en bloc chest wall, thymus, and heart transplant model in mice using a cervical non-suture cuff technique.

Medicine

Development of a Selective Aortic Arch Perfusion System in a Porcine Model of Exsanguination Cardiac Arrest

Marta J. Madurska¹, Hossam Abdou¹, Michael J. Richmond¹, Noha N. Elansary¹, Peng F. Wong², Todd E. Rasmussen³, Thomas M. Scalea¹, Jonathan J. Morrison¹

¹R Adams Cowley Shock Trauma Center, ²Department of Vascular Surgery, The James Cook University Hospital, ³Uniformed Services University of the Health Sciences

The goal of this protocol is to demonstrate a porcine exsanguination cardiac arrest model and a specifically built selective aortic arch perfusion circuit for translational research.