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National Taiwan University College of Medicine

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
Shiun Chang 1, Li-Mei Pai 2,3, Chien-Kuo Lee 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Biochemistry, Chang Gung University College of Medicine, 3Molecular Medicine Research Center, Chang Gung University College of Medicine

In this study we present an in vitro culture system that can efficiently generate pDCs by co-culturing common lymphoid progenitors with AC-6 feeder cells in the presence of Flt3 ligand.

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Immunology and Infection

Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research
Yu-Jei Lin 1, Tsung-Lin Lee 1, Chia-Chi Ku 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine

This protocol is a cost-effective alternative for expressing naked plasmid DNA in mouse skin. The overall goal of the protocol is to deliver immune-related genes into skin tissue to delineate the functional role of a specific gene in cutaneous inflammation.

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Immunology and Infection

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
Yu-Ling Hsiao 1, Hans Häcker 2, Chien-Kuo Lee 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology, University of Utah

Here we provide a protocol for screening potential transcription factors involved in the development of dendritic cell (DC) using lentiviral transduction of shRNA to obtain stable knockdown cell lines for in vitro DC differentiation.

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Immunology and Infection

A Clinically Relevant Murine Model of Peritoneal Fibrosis by Dialysate and Catheters
Yi-Ting Chen 1,2,3, Shuei-Liong Lin 1,2,3
1Department of Integrated Diagnostics & Therapeutics, National Taiwan University Hospital, 2Renal Division, Department of Internal Medicine, National Taiwan University Hospital, 3Graduate Institute of Physiology, National Taiwan University College of Medicine

Patients with severe peritoneal fibrosis have high morbidity and mortality. The mechanism of peritoneal fibrosis is unclear. In this study, we describe a simple experimental murine model of peritoneal fibrosis induced by the injection of peritoneal dialysis fluid.

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