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Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

3.4K Views

06:12 min

March 7th, 2022

DOI :

10.3791/62730-v

March 7th, 2022


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Dendritic Cells

Chapters in this video

0:04

Introduction

0:43

Preparation of Immortalized HematopoieticStem and Progenitor Cell (iHSPC) Lines and Lentiviral Transduction

2:08

In Vitro Differentiation of the Stable Knockdown iHSPC Cell Lines

3:05

Flow Cytometric Analysis of the Differentiated Dendritic Cells

4:01

Results: Dendritic Cell Differentiation Following Short Hairpin RNA-Mediated Tcf4 and Id2 Knockdown

5:33

Conclusion

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