Jiangsu University

5 ARTICLES PUBLISHED IN JoVE

Biology

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

¹Department of Physiology, Jules Stein Eye Institute and Howard Hughes Medical Institute, University of California, Los Angeles

Here we describe an optimized technique to produce high-quality vitamin A/RBP complex and two real-time monitoring techniques to study vitamin A transport by STRA6, the RBP receptor.

Medicine

Intra-Operative Neural Monitoring of Thyroid Surgery in a Porcine Model

Che-Wei Wu^1,2,3, Tzu-Yen Huang², Hui-Chun Chen⁴, Hsiu-Ya Chen⁵, Tsung-Yi Tsai², Pi-Ying Chang⁵, Yi-Chu Lin², Hsin-Yi Tseng², Pao-Chu Hun⁶, Xiaoli Liu⁷, Hui Sun⁷, Gregory W. Randolph⁸, Gianlorenzo Dionigi⁹, Feng-Yu Chiang^2,3, I-Cheng Lu^5,10,11

This study aims to develop a standard protocol of intra-operative neural monitoring of thyroid surgery in a porcine model. Here, we present a protocol to demonstrate general anesthesia, to compare different types of electrodes, and to investigate the electrophysiological characteristics of the normal and injured recurrent laryngeal nerves.

Bioengineering

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters

Yong M. Lyu¹, Yu Q. Li¹, Hui B. Song¹, Juan Guo¹, Ting Wang¹, Li Liu¹, Gabriel Yedid², Josef Voglmeir¹

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, ²College of Life Science, Nanjing Agricultural University

We describe a strategy for how to use RNA samples from unreferenced Pacific oyster specimens, and evaluate the genetic material by comparison with publicly available genome data to generate a virtually sequenced cDNA library.

Bioengineering

Bioinspired Soft Robot with Incorporated Microelectrodes

Ting Wang^1,2, Bianca Migliori^1,3, Beatrice Miccoli^1,4, Su Ryon Shin¹

¹Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, ²School of Medicine, Jiangsu University, ³Tech4Health and Neuroscience Institutes, NYU Langone Health, ⁴Department of Electronics and Telecommunication, Politecnico di Torino

A bioinspired scaffold is fabricated by a soft photolithography technique using mechanically robust and electrically conductive hydrogels. The micropatterned hydrogels provide directional cardiomyocyte cell alignment, resulting in a tailored direction of actuation. Flexible microelectrodes are also integrated into the scaffold to bring electrical controllability for a self-actuating cardiac tissue.

JoVE Core

Direct Reprogramming of Mouse Fibroblasts into Melanocytes

Yi-Xuan Zhang^1,2, Li-Ping Liu^1,2, Ming Jin^1,2, Hui Sun^1,2, Han-Lin Zhang^1,2, Yu-Mei Li^1,2

¹Institute for Regenerative Medicine, Jiangsu University, ²Department of Dermatology, Affiliated Hospital of Jiangsu University

Here, we describe an optimized direct reprogramming system for melanocytes and a high-efficiency, concentrated virus packaging system that ensures smooth direct reprogramming.