Anhui Agricultural University

4 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats

Shihai Jiang^*1, Xiaoyou Yi^*3, Yuansen Luo², Dongjie Yu¹, Yuangao Liu¹, Fei Zhang^1,4, Lei Zhu², Kun Wang¹

¹Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, ²Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, ³Department of Bone Surgery, Tungwah Hospital of Sun Yat-sen University, ⁴Department of Othopaedic Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University

Here, we present a protocol to describe a minimally invasive technique for knee joint immobilization in a rat model. This reproducible protocol, basing on muscle-gap separation modus and the mini-incision skill, is suitable for studying the underlying molecular mechanism of acquired joint contracture.

Biochemistry

Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves

Weiting Jiao^*1,2, Guoqin Ge^*1, Rimao Hua², Jun Sun¹, Yeyun Li¹, Ruyan Hou¹

¹State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Province Key Lab of Analysis and Detection for Food Safety, ²School of Resource & Environment, Anhui Agricultural University, Key Laboratory of Agri-food Safety of Anhui Province

This work presents a protocol for establishing a cell suspension culture derived from tea (Camellia sinensis L.) leaves that can be used to study the metabolism of external compounds that can be taken up by the whole plant, such as insecticides.

Environment

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples

Weiting Jiao^1,2, Tingting Shen², Luyao Wang¹, Lei Zhu^1,3, Qing X. Li⁴, Chen Wang³, Hongping Chen³, Rimao Hua¹, Xiangwei Wu¹

¹Key Laboratory of Agri-food Safety of Anhui Province, School of Resource & Environment, Anhui Agricultural University, ²State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, ³Key Laboratory of Tea Quality and Safety & Risk Assessment, Tea Research Institute, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, ⁴Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa

The present protocol describes the contamination of pyrrolizidine alkaloids (PAs) in tea samples from PA-producing weeds in tea gardens.

Chemistry

Tea Aroma Analysis Based on Solvent-Assisted Flavor Evaporation Enrichment

Zhihui Feng¹, Xiaogen Yang², Chun Zou¹, Junfeng Yin¹

¹Tea Research Institute Chinese Academy of Agricultural Sciences, ²State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University

Presented here is a method for enriching and analyzing the volatile components of tea extracts using solvent-assisted flavor evaporation and solvent extraction followed by gas chromatography-mass spectrometry, which can be applied to all types of tea samples.