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Swiss Institute of Bioinformatics

3 ARTICLES PUBLISHED IN JoVE

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Environment

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Tina Wunderlin 1, Thomas Junier 2, Christophe Paul 3, Nicole Jeanneret 3, Pilar Junier 3
1Department of Biological Sciences, Macquarie University, 2Vital-IT group, Swiss Institute of Bioinformatics, 3Laboratory of Microbiology, University of Neuchatel

A method to single out bacterial endospores from complex microbial communities was developed to perform tailored culture or molecular studies of this group of bacteria.

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Chemistry

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
Mohameedyaseen Syedbasha 1, Janina Linnik 1,2,3, Deanna Santer 4, Daire O'Shea 5, Khaled Barakat 4,6, Michael Joyce 4, Nina Khanna 7, D. Lorne Tyrrell 4, Michael Houghton 4, Adrian Egli 1,8
1Applied Microbiology Research, Department of Biomedicine, University of Basel, 2Department of Biosystems Science and Engineering, ETH Zurich, and Swiss Institute of Bioinformatics, 3Swiss Institute of Bioinformatics, 4Li Ka Shing Institute for Virology, University of Alberta, 5Regional Infectious Diseases Unit, University of Edinburgh, 6Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 7Infection Biology, Department of Biomedicine, University of Basel, 8Clinical Microbiology, University Hospital Basel

The presented protocols describe two enzyme-linked immunosorbent assay (ELISA) based techniques for the rapid investigation of ligand-receptor interactions: The first assay allows the determination of dissociation constant between ligand and receptor. The second assay enables a rapid screening of blocking peptides for ligand-receptor interactions.

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Medicine

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
Lukas Kaufmann *1, Mohammedyaseen Syedbasha *1, Dominik Vogt 1, Yvonne Hollenstein 1, Julia Hartmann 1, Janina E. Linnik 1,2,3, Adrian Egli 1,4
1Applied Microbiology Research, Department of Biomedicine, University of Basel, 2Department of Biosystems Science and Engineering, ETH Zurich, 3Swiss Institute of Bioinformatics, 4Clinical Microbiology, University Hospital Basel

The presented protocols describe how to perform a hemagglutination inhibition assay to quantify influenza-specific antibody titers from serum samples of influenza vaccine recipients. The first assay determines optimal viral antigen concentrations by hemagglutination. The second assay quantifies influenza-specific antibody titers by hemagglutination inhibition.

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