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Swiss Institute of Bioinformatics

3 ARTICLES PUBLISHED IN JoVE

Environment

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells

Tina Wunderlin¹, Thomas Junier², Christophe Paul³, Nicole Jeanneret³, Pilar Junier³

¹Department of Biological Sciences, Macquarie University, ²Vital-IT group, Swiss Institute of Bioinformatics, ³Laboratory of Microbiology, University of Neuchatel

A method to single out bacterial endospores from complex microbial communities was developed to perform tailored culture or molecular studies of this group of bacteria.

Chemistry

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

Mohameedyaseen Syedbasha¹, Janina Linnik^1,2,3, Deanna Santer⁴, Daire O'Shea⁵, Khaled Barakat^4,6, Michael Joyce⁴, Nina Khanna⁷, D. Lorne Tyrrell⁴, Michael Houghton⁴, Adrian Egli^1,8

¹Applied Microbiology Research, Department of Biomedicine, University of Basel, ²Department of Biosystems Science and Engineering, ETH Zurich, and Swiss Institute of Bioinformatics, ³Swiss Institute of Bioinformatics, ⁴Li Ka Shing Institute for Virology, University of Alberta, ⁵Regional Infectious Diseases Unit, University of Edinburgh, ⁶Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, ⁷Infection Biology, Department of Biomedicine, University of Basel, ⁸Clinical Microbiology, University Hospital Basel

The presented protocols describe two enzyme-linked immunosorbent assay (ELISA) based techniques for the rapid investigation of ligand-receptor interactions: The first assay allows the determination of dissociation constant between ligand and receptor. The second assay enables a rapid screening of blocking peptides for ligand-receptor interactions.

Medicine

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

Lukas Kaufmann^*1, Mohammedyaseen Syedbasha^*1, Dominik Vogt¹, Yvonne Hollenstein¹, Julia Hartmann¹, Janina E. Linnik^1,2,3, Adrian Egli^1,4

¹Applied Microbiology Research, Department of Biomedicine, University of Basel, ²Department of Biosystems Science and Engineering, ETH Zurich, ³Swiss Institute of Bioinformatics, ⁴Clinical Microbiology, University Hospital Basel

The presented protocols describe how to perform a hemagglutination inhibition assay to quantify influenza-specific antibody titers from serum samples of influenza vaccine recipients. The first assay determines optimal viral antigen concentrations by hemagglutination. The second assay quantifies influenza-specific antibody titers by hemagglutination inhibition.