University of Szeged

6 ARTICLES PUBLISHED IN JoVE

Neuroscience

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Marie Vandecasteele^1,2, S. M.¹, Sébastien Royer^1,3, Mariano Belluscio¹, Antal Berényi¹, Kamran Diba^1,4, Shigeyoshi Fujisawa¹, Andres Grosmark¹, Dun Mao¹, Kenji Mizuseki¹, Jagdish Patel¹, Eran Stark¹, David Sullivan¹, Brendon Watson¹, György Buzsáki¹

¹Center for Molecular and Behavioral Neuroscience, University of New Jersey, ²Center for Interdisciplinary Research in Biology, Collège de France, ³Janelia Farm Research Campus, Howards Hughes Medical Institute, ⁴Deptartment of Psychology, University of Wisconsin at Milwaukee

We describe methods for large-scale recording of multiple single units and local field potential in behaving rodents with silicon probes. Drive fabrication, probe attachment to the drive and probe implantation processes are illustrated in sufficient details for easy replication.

Biology

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Yang Song^*1, Xuemei Zhang^*1, Lingyan Huang², Mark A. Behlke², Andrew Tsourkas¹

¹Department of Bioengineering, University of Pennsylvania, ²Integrated DNA Technologies, Inc.

Ratiometric bimolecular beacons (RBMBs) can be used to image single engineered RNA transcripts in living cells. Here, we describe the preparation and purification of RBMBs, delivery of RBMBs into cells by microporation and fluorescent imaging of single RNA transcripts in real-time.

Neuroscience

Chronic Transcranial Electrical Stimulation and Intracortical Recording in Rats

Gábor Kozák¹, Tamás Földi¹, Antal Berényi^1,2

¹MTA-SZTE "Momentum" Oscillatory Neuronal Networks Research Group, Department of Physiology, University of Szeged, ²New York University Neuroscience Institute, New York University

This detailed protocol describes transcranial stimulation electrode placement on the temporal bone in order to investigate the short- and long-term effects of transcranial electrical stimulation in freely moving rats.

Neuroscience

Electrophoretic Delivery of γ-aminobutyric Acid (GABA) into Epileptic Focus Prevents Seizures in Mice

Andrea Slezia^*1,5, Christopher M. Proctor^*2,3, Attila Kaszas^1,4, George G. Malliaras^2,3, Adam Williamson^1,5

¹Aix Marseille Université, Institut de Neurosciences des Systèmes (INS), ²Electrical Engineering Division, University of Cambridge, ³Department of Bioelectronics, Centre Microélectronique de Provence - Ecole Nationale Supérieure des Mines de Saint-Étienne (CMP-EMSE), ⁴Institut de Neurosciences de la Timone, Centre National de la Recherche Scientifique (CNRS) UMR 7289 & Aix- Marseille Université, ⁵Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged

The challenge of epilepsy research is to develop novel treatments for patients where classical therapy is inadequate. Using a new protocol—with the help of an implantable drug delivery system—we are able to control seizures in anesthetized mice by the electrophoretic delivery of GABA into the epileptic focus.

JoVE Core

Lower-Limb Biomechanical Characteristics Associated with Unplanned Gait Termination Under Different Walking Speeds

Huiyu Zhou^1,2, Xuanzhen Cen¹, Yang Song³, Ukadike C. Ugbolue², Yaodong Gu¹

¹Faculty of Sports Science, Ningbo University, ²School of Health and Life Sciences, University of the West of Scotland, ³Faculty of Engineering, University of Szeged

This study compared the biomechanical characteristics of the lower extremity during unplanned gait termination under different walking speeds. The lower-limb kinematic and kinetic data from fifteen subjects with normal and fast walking speeds were collected using a motion analysis system and plantar pressure platform.

Biology

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Vasiliki Pantazi^*1, Ivett Berzsenyi^*1, Barbara N. Borsos¹, Tibor Pankotai¹

¹Institute of Pathology, Faculty of Medicine, University of Szeged

This article introduces essential steps of immunostaining and chromatin immunoprecipitation. These protocols are commonly used to study DNA damage-related cellular processes and to visualize and quantify the recruitment of proteins implicated in DNA repair.