UNIVERSIDAD IBEROAMERICANA Ciudad de México

2 ARTICLES PUBLISHED IN JoVE

Biology

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Mario Buenrostro-Jauregui^*1, Alejandro Tapia-de-Jesús¹, Jesus Mata¹, Luis Miguel Rodríguez-Serrano¹, María Elena Chávez-Hernández¹, Florencia Mata¹, Marco Monroy-Plasencia^1,2, Aixchell Camila Alonso-Flores¹, Perla Leal-Galicia^*1

¹Laboratorio de Neurociencias, Departamento de Psicología, Universidad Iberoamericana Ciudad de México, ²Facultad de Ciencias, Universidad Autónoma del Estado de México

This paper presents four of the most common techniques for visualizing BrdU positive cells to measure adult neurogenesis in rats. This work includes instructions for reagent preparation, thymidine analog administration, transcardial perfusion, tissue preparation, peroxidase immunohistochemical reaction, immunofluorescence, signal amplification, counterstaining, microscopy imaging, and cell analysis.

Behavior

Simultaneous Monitoring of Wireless Electrophysiology and Memory Behavioral Test as a Tool to Study Hippocampal Neurogenesis

Mario Buenrostro-Jáuregui^*1, Luis Miguel Rodríguez-Serrano¹, María Elena Chávez-Hernández¹, Alejandro Tapia-de-Jesús¹, Jesus Mata-Luevanos¹, Florencia Mata¹, Oscar Galicia-Castillo¹, Daniel Tirado-Martínez¹, Sylvia Ortega-Martinez², Erik Bojorges-Valdez^*3

¹Laboratorio de Neurociencias, Departamento de Psicología, Universidad Iberoamericana Ciudad de México, ²Stoeling Co., ³Departamento de Estudios en Ingeniería para la Innovación, Universidad Iberoamericana Ciudad de México

The protocol presented here provides information on the simultaneous electroencephalography (EEG) and behavioral assessment in real-time. We have discussed all steps involved in this protocol as an attractive solution for researchers in many fields of neuroscience, particularly in learning and memory areas.