Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

7.4K Views

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08:37 min

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September 9th, 2020

DOI :

10.3791/61483-v

September 9th, 2020

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Mario Buenrostro-Jauregui*¹, Alejandro Tapia-de-Jesús¹, Jesus Mata¹, Luis Miguel Rodríguez-Serrano¹, María Elena Chávez-Hernández¹, Florencia Mata¹, Marco Monroy-Plasencia¹^,², Aixchell Camila Alonso-Flores¹, Perla Leal-Galicia*¹

¹Laboratorio de Neurociencias, Departamento de Psicología, Universidad Iberoamericana Ciudad de México, ²Facultad de Ciencias, Universidad Autónoma del Estado de México

Summary

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Immunohistochemistry

Cellular Proliferation

Protein Co expression

Membrane Permeabilization

Avidin biotin peroxidase Complex

Blocking Solution

Chapters in this video

0:04

Introduction

1:06

Thymidine Analog (5‐Bromo‐2'‐Deoxyuridine) BrdU Administration and Tissue Preparation

2:02

BrdU Detection

4:40

Cell Quantification

5:33

Image Deconvolution

6:59

Results: BrdU‐Labeled Cell Identification and Quantification in Representative DG Tissue Section

7:46

Conclusion

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