JoVE Logo

Sign In

Van Andel Research Institute

4 ARTICLES PUBLISHED IN JoVE

image

Biology

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Chen Lu 1, Joshua L. Wonsidler 1, Jianwei Li 2, Yanming Du 1, Timothy Block 3, Brian Haab 4, Songming Chen 1
1Institute for Hepatitis and Virus Research, 2Department of Microbiology and Immunology, Thomas Jefferson University, 3Drexel University College of Medicine, 4Van Andel Research Institute

In this study, we describe an improved protocol for a multiplexed high-throughput antibody microarray with lectin detection method that can be used in glycosylation profiling of specific proteins. This protocol features new reliable reagents and significantly reduces the time, cost, and lab equipment requirements as compared to the previous procedure.

image

Biochemistry

Analysis of Histone Antibody Specificity with Peptide Microarrays
Evan M. Cornett 1, Bradley M. Dickson 1, Scott B. Rothbart 1
1Center for Epigenetics, Van Andel Research Institute

This manuscript describes methods for applying peptide microarray technology to specificity profiling of antibodies that recognize histones and their post-translational modifications.

image

Genetics

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Nicole A. Vander Schaaf 1, Shirley Oghamian 2, Jin-A Park 1, Liang Kang 1, Peter W. Laird 1, Kwang-Ho Lee 1
1Center for Epigenetics, Van Andel Research Institute, 2Amgen

This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator systems.

image

Cancer Research

Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models
Elizabeth Kenyon 1,2, Erin K. Zaluzec 1,3, Katherine Powell 1,2, Maximilian Volk 1,4, Shatadru Chakravarty 2,5, Jeremy Hix 2,6, Ripla Arora 7,8, Jennifer J. Westerhuis 9,10, Matti Kiupel 11, Erik M. Shapiro 2,6, Lorenzo F. Sempere 1,2
1Precision Health Program, Michigan State University, 2Department of Radiology, College of Human Medicine, Michigan State University, 3Department of Pharmacology & Toxicology, College of Veterinary Medicine, Michigan State University, 4College of Osteopathic Medicine, Michigan State University, 5Advanced Materials Characterization Laboratory/Materials Research Center, Missouri University of Science and Technology, 6Institute for Quantitative (IQ) Health Science and Engineering Advanced Molecular Imaging Facility, Michigan State University, 7Department of Obstetrics Gynecology and Reproductive Biology, College of Human Medicine, Michigan State University, 8Institute for Quantitative (IQ) Health Science and Engineering, Michigan State University, 9Van Andel Research Institute, 10Miltenyi Biotec, 11Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Michigan State University

A method of intraductal injection of reagents for an ethanol-based ablative solution to the mouse mammary ductal tree for in vivo imaging and breast cancer prevention is described. Injection directly into the nipple opening allows for targeting mammary epithelial cells with minimal collateral tissue damage.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved