Inactivation of mTor: A Tool to Investigate Meiotic Progression and Translational Control During Bovine Oocyte MaturationManuela Kreißelmeier 1, Sophia Mayer 1, Christine Wrenzycki 2, Ralf Pöhland 3, Wolfgang Tomek 4
1BVN Neustadt/Aisch, 2Faculty of Veterinary Medicine, Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University Giessen, 3Reproductive Cell Biology Unit, Leibniz Institute for Farm Animal Biology, 4Reproductive Biochemistry Unit, Leibniz Institute for Farm Animal Biology
The protocol presented here describes the inhibition of the Ser/Thr kinase mTor during IVM of bovine oocytes. This approach can facilitate the investigation of meiotic progression and translational control. It also contributes to the definition of developmental competence and the improvement of IVM conditions.
