Justus-Liebig-University Giessen

1 ARTICLES PUBLISHED IN JoVE

Biology

Inactivation of mTor: A Tool to Investigate Meiotic Progression and Translational Control During Bovine Oocyte Maturation

Manuela Kreißelmeier¹, Sophia Mayer¹, Christine Wrenzycki², Ralf Pöhland³, Wolfgang Tomek⁴

¹BVN Neustadt/Aisch, ²Faculty of Veterinary Medicine, Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University Giessen, ³Reproductive Cell Biology Unit, Leibniz Institute for Farm Animal Biology, ⁴Reproductive Biochemistry Unit, Leibniz Institute for Farm Animal Biology

The protocol presented here describes the inhibition of the Ser/Thr kinase mTor during IVM of bovine oocytes. This approach can facilitate the investigation of meiotic progression and translational control. It also contributes to the definition of developmental competence and the improvement of IVM conditions.