Capital Normal University

4 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Method for Characterizing Embryogenesis in Arabidopsis

¹College of Life Sciences, Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Capital Normal University, ²College of Life Science, Shanxi Normal University

This protocol outlines a method for observing embryogenesis in Arabidopsis via ovule clearance followed by the inspection of embryo pattern formation under a microscope.

Chemistry

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Xiao Wu^*1, Donglin Diao^*1, Zhangwei Lu^*1, Yu Han¹, Shi Xu², Xinhui Lou¹

¹Department of Chemistry, Capital Normal University, ²College of Life Sciences, Capital Normal University

A protocol for the in vitro selection and characterization of group-specific phthalic acid ester- binding DNA aptamers is presented. The application of the selected aptamer in an electrochemical aptasensor is also included.

Behavior

An Adjustable High-Definition Imaging System for Behavioral Studies of Drosophila Adults

Tong Li^*1,2, Yujia Weng^*1,2, Daxiang Yang¹

¹Department of Zoology and Animal Physiology, College of Biological Sciences, China Agricultural University, ²College of Life Sciences, Capital Normal University

This protocol describes how to make a simple adult Drosophila behavior observation chamber, and how to take high-definition photographs/videos of the morphology or behavior of different types of fruit flies in the observation chamber through relatively simple and affordable methods.

Biochemistry

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

Yu Han^1,3, Cody T. Thomas^1,3, Sara A. Wennersten^1,3, Edward Lau^1,3, Maggie P. Y. Lam^1,2,3

¹Department of Medicine-Cardiology, University of Colorado Anschutz Medical Campus, ²Department of Biochemistry & Molecular Genetics, University of Colorado Anschutz Medical Campus, ³Consortium for Fibrosis Research & Translation, School of Medicine, University of Colorado Anschutz Medical Campus

Detailed protocol and three Python scripts are provided for operating an open-source robotic liquid handling system to perform semi-automated protein sample preparation for mass spectrometry experiments, covering detergent removal, protein digestion, and peptide desalting steps.