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JoVE Journal

Chemistry

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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

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09:33 min

March 21st, 2018

March 21st, 2018

9,722 Views

0:04

Title

0:50

Small-scale λ Exonuclease Reaction

2:40

Relative Affinity and Specificity Test by Competitive Assays

4:17

Fabrication and Electrochemical Measurements of Diethylhexyl Phthalate (DEHP) Electrochemical Biosensors

7:52

Results: Optimization of λ Exonuclease Reaction and Electrochemical Aptasensor Evaluation

8:46

Conclusion

Transcript

The overall goal of this procedure is to select group-specific DNA aptamers to highly hydrophobic, small molecules and to use the selected aptamer to develop a sensitive electrochemical biosensor. This method can help to answer key questions in th

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A protocol for the in vitro selection and characterization of group-specific phthalic acid ester- binding DNA aptamers is presented. The application of the selected aptamer in an electrochemical aptasensor is also included.

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