Huaqiao University

3 ARTICLES PUBLISHED IN JoVE

Biology

In situ Hybridization for Sipunculus nudus Coelomic Fluid

¹Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences, Huaqiao University

This protocol describes an effective in situ hybridization approach to detect the mRNA expression levels and spatial patterns of target genes in Sipunculus nudus coelomic fluid.

Bioengineering

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology

Sheng-Chang Luo^1,2, Ying Wang³, Ranjith Kumar Kankala^1,2, Yu Shrike Zhang⁴, Ai-Zheng Chen^1,2

¹Institute of Biomaterials and Tissue Engineering, Huaqiao University, ²Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, ³Affiliated Dongguan Hospital, Southern Medical University, ⁴Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School

The present protocol describes the fabrication of poly(lactic-co-glycolic acid)-based highly open porous microspheres (HOPMs) via the single-emulsion formulation based facile microfluidic technology. These microspheres have potential applications in tissue engineering and drug screening.

Biochemistry

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Mingqing Tang^1,2, Hongjun Lin¹, Chengjia Hu¹, Hui Yan^1,2

¹Medicine School, Huaqiao University, ²Engineering Research Centre of Molecular Medicine of Ministry of Education

Here, we present an affinity purification method of a fibrinolytic enzyme from Sipunculus nudus that is simple, inexpensive, and efficient.