Inner Mongolia Medical University

6 ARTICLES PUBLISHED IN JoVE

Biology

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Xin Zhang^*1, Keshava Mysore^*2,3, Ellen Flannery^3,4, Kristin Michel¹, David W. Severson^3,4, Kun Yan Zhu⁵, Molly Duman-Scheel^2,3,4

¹Division of Biology, Kansas State University, ²Department of Medical and Molecular Genetics, Indiana University School of Medicine, ³Eck Institute for Global Health, University of Notre Dame, ⁴Department of Biological Sciences, University of Notre Dame, ⁵Department of Entomology, Kansas State University

Here we describe a procedure for inhibiting gene function in disease vector mosquitoes through the use of chitosan/interfering RNA nanoparticles that are ingested by larvae.

Cancer Research

Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis

Ravan Moret¹, Linh Hellmers¹, Xin Zhang¹, Jessie Gills², Nathan Hite³, Aaron Klinger³, Grace A. Maresh¹, Daniel Canter^2,4, Stephen Bardot^2,4, David A Margolin^3,4, Li Li^1,4

¹Laboratory of Translational Cancer Research, Institute for Translational Research, Ochsner Clinic Foundation, ²Department of Urology, Ochsner Clinic Foundation, ³Department of Colon and Rectal Surgery, Ochsner Clinic Foundation, ⁴Ochsner Clinical School, University of Queensland, School of Medicine

This protocol describes the generation of patient-derived orthotopic xenograft models by intra-vesically instilling high-grade urothelial cell carcinoma cells or intra-rectally injecting colorectal cancer cells into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice for primary tumor growth and spontaneous metastases under the influence of lymph node stromal cells, which mimics the progression of human metastatic diseases.

Behavior

Physical Activity Measurement in Children Accepting Table Tennis Training

Xin Zhang^*1, Cuiqiao Xia^*2, Xiuli Zhao³, Yu Liu³, Hóngyi Zhào^2,3, Yonghua Huang²

¹Department of Sport, Inner Mongolia Medical University, ²Department of Neurology, The Seventh Medical Center of PLA General Hospital, ³Department of Neurology, NO 984 Hospital of PLA

This study proposes an accelerometer-based method to objectively measure physical activity (PA) and leisure time physical activity (LTPA) in Chinese children accepting table tennis training in clubs.

Medicine

Behavioral and Network Pharmacology-Based Analyses for the Traditional Mongolian Medicine Zadi-5 in a Rat Model of Depression

Riga Wu^*1, Wen Zu^*1, Lisi Wu¹, Si Su¹, Nier Su¹, Ligeer Qi¹, Rilaga Wu¹, Wei Sun², Rilebagen Hu¹

¹Faculty of Mongolian Medicine, Inner Mongolia Medical University, ²Affiliated Hospital of Inner Mongolia Medical University

The present protocol describes a method for the behavioral test validation and bioinformatical prediction of the therapeutic efficacy of Zadi-5, a traditional Mongolian medicine, in depression.

Medicine

Chronic Unpredictable Mild Stress in Rats based on the Mongolian medicine

Hongmei Xin^*1, Si Su^*2, Riga Wu², Lisi Wei², Nier Su², Ligeer Qi², Rilaga Wu², Ruhan A², Lijun Tong², Wuji Wang³, Jing Zhang³, Rilebagen Hu², Li Li³

¹Faculty of Humanities Education, Inner Mongolia Medical University, ²Faculty of Mongolian Medicine, Inner Mongolia Medical University, ³Faculty of Basic Medical, Inner Mongolia Medical University

This protocol outlines a chronic unpredictable mild stress (CUMS) model for depression based on Mongolian medical theory, along with methods for validating behavioral tests.

Biology

In Vitro Modeling of Fat Deposition in Metabolic Dysfunction-Associated Steatotic Liver Disease

Qinglan Bao¹, Xiaoxiao Zhang², Yingsong Chen^*1,3,4, Tegexibaiyin Wang^*5, Bateer Siqin^*6

¹Inner Mongolia Medical University, ²Xilin Gol League Mongolian Medical Hospital, ³National and local Joint Mongolian Medicine R & D Engineering Center, ⁴Inner Mongolia Minzu University, ⁵Affiliated Hospital of Inner Mongolia Minzu University, ⁶Traditional Chinese & Mongolian Medical Research Institute of Inner Mongolia

This article describes the use of oleic acid-induced HepG2 cells as a model for metabolic dysfunction-associated steatotic liver disease.