Wuyi University

4 ARTICLES PUBLISHED IN JoVE

Medicine

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Dongshan Yang¹, Jifeng Zhang¹, Jie Xu¹, Tianqing Zhu¹, Yanbo Fan¹, Jianglin Fan², Y. Eugene Chen¹

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, ²Department of Molecular Pathology, University of Yamanashi

Recent development in gene targeting tools makes production of knockout (KO) rabbits possible. In the present work, we generated five Apolipoprotein (Apo) C-III KO rabbits using Zinc Finger Nucleases (ZFN). This work demonstrated that ZFN is a highly efficient method to produce KO rabbits.

Biochemistry

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Manabu Niimi¹, Haizhao Yan¹, Yajie Chen¹, Yao Wang², Jianglin Fan^1,2

¹Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine, University of Yamanashi, ²School of Biotechnology and Health Sciences, Wuyi University

Several methods have been used for analyzing plasma lipoproteins; however, ultracentrifugation is still one of the most popular and reliable methods. Here, we describe a method regarding how to isolate lipoproteins from plasma using sequential density ultracentrifugation and how to analyze the apolipoproteins for both diagnostic and research purposes.

Bioengineering

Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers

Lara Hairapetian¹, Enrico Cortes¹, Junyi Zhao¹, Yao Wang¹, Ricky Huang¹, Robert Damoiseaux^1,2, Ivan Pushkarsky¹

¹Forcyte Biotechnologies, ²University of California, Los Angeles

This work presents a flexible protocol for utilizing fluorescently labeled elastomeric contractible surfaces (FLECS) Technology in microwell format for simplified, hands-off quantification of single-cell contractile forces based on visualized displacements of fluorescent protein micropatterns.

Bioengineering

Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

Bangzhu Chen^1,2, Qinrong Lin^1,2, Nengqing Liu^1,2, Diyu Chen^1,2, Yinghui Zhang³, Xiaofang Sun^1,2

¹Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, ²Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, ³School of Biotechnology and Health Sciences, Wuyi University

Cerebral organoids provide unprecedented opportunities for studying organ development and human disease pathology. Although great success has been achieved with cerebral organoid culture systems, there are still operational difficulties in applying this technology. The present protocol describes a cerebral organoid procedure that facilitates medium change and organoid transfer.