JoVE Logo

Sign In

Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

2.2K Views

09:10 min

June 6th, 2022

DOI :

10.3791/63989-v

June 6th, 2022


Explore More Videos

Cerebral Organoid Culture

Chapters in this video

0:04

Introduction

0:51

Induced Pluripotent Stem Cells (iPSCs) and EB Preparation (Days 0 1)

2:54

Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)

4:24

Neural Induction (Days 5 7)

5:25

Embedding in the Basement Membrane Matrix (Days 7 10)

6:52

Organoid Maturation (Days 10 40)

7:21

Results: Analysis of Cerebral Organoids

8:41

Conclusion

Related Videos

article

10:25

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

21.2K Views

article

07:11

Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture

3.1K Views

article

14:19

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

3.8K Views

article

09:36

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases

9.8K Views

article

08:20

Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research

3.2K Views

article

08:58

A Human Cerebral Organoid Model of Neural Cell Transplantation

1.0K Views

article

07:42

A High-Throughput Platform for Culture and 3D Imaging of Organoids

2.4K Views

article

01:20

Generating an Organoid Culture from a Brain Tumor Cell Suspension

173 Views

article

01:13

A Technique to Optimize Cerebral Organoid Culture Using Lateral Soft Light Illumination

20 Views

article

04:19

Generating Induced Pluripotent Stem Cell-Derived Cerebral Organoids

53 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved