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University of Electronic Science and Technology of China

6 ARTICLES PUBLISHED IN JoVE

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Chemistry

Low Pressure Vapor-assisted Solution Process for Tunable Band Gap Pinhole-free Methylammonium Lead Halide Perovskite Films
Carolin M. Sutter-Fella *1,2,3, Yanbo Li *1,4, Nicola Cefarin 1,5,6, Aya Buckley 1,7, Quynh Phuong Ngo 8,9, Ali Javey 2,3, Ian D. Sharp 1, Francesca M. Toma 1
1Joint Center for Artificial Photosynthesis, Chemical Sciences Division, Lawrence Berkeley National Laboratory, 2Electrical Engineering and Computer Sciences, University of California, Berkeley, 3Materials Science Division, Lawrence Berkeley National Laboratory, 4Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 5Department of Physics, Graduate School of Nanotechnology, University of Trieste, 6TASC Laboratory, IOM-CNR - Istituto Officina dei Materiali, 7Department of Chemistry, University of California, Berkeley, 8Materials Science and Engineering, University of California, Berkeley, 9Joint Center for Artificial Photosynthesis, Lawrence Berkeley National Laboratory

Here, we present a protocol for the synthesis of CH3NH3I and CH3NH3Br precursors and the subsequent formation of pinhole-free, continuous CH3NH3PbI3-xBrx thin films for the application in high efficiency solar cells and other optoelectronic devices.

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JoVE Core

Generation and Coherent Control of Pulsed Quantum Frequency Combs
Benjamin MacLellan *1, Piotr Roztocki *1, Michael Kues 1,2, Christian Reimer 1, Luis Romero Cortés 1, Yanbing Zhang 1, Stefania Sciara 1,3, Benjamin Wetzel 1,4, Alfonso Cino 3, Sai T. Chu 5, Brent E. Little 6, David J. Moss 7, Lucia Caspani 8, José Azaña 1, Roberto Morandotti 1,9,10
1Institut National de la Recherche Scientifique - Centre Énergie, Matériaux et Télécommunications (INRS-EMT), 2School of Engineering, University of Glasgow, 3Department of Energy, Information Engineering and Mathematical Models, University of Palermo, 4School of Mathematical and Physical Sciences, University of Sussex, 5Department of Physics and Material Science, City University of Hong Kong, 6State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Science, 7Centre for Micro Photonics, Swinburne University of Technology, 8Institute of Photonics, Department of Physics, University of Strathclyde, 9Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 10National Research University of Information Technologies, Mechanics and Optics

A protocol is presented for the practical generation and coherent manipulation of high-dimensional frequency-bin entangled photon states using integrated micro-cavities and standard telecommunications components, respectively.

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Immunology and Infection

Enhancement Method of Surface Acoustic Wave-Atomizer Efficiency for Olfactory Display
Takamichi Nakamoto 1, Sami Ollila 1, Shingo Kato 1, Haining Li 1,2, Guiping Qi 1
1Tokyo Institute of Technology, 2University of Electronic Science and Technology of China

We establish here a method for coating the surface of a surface acoustic wave (SAW) device with amorphous Teflon film to improve the atomization efficiency required for application to an olfactory display.

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Developmental Biology

Monocular Visual Deprivation and Ocular Dominance Plasticity Measurement in the Mouse Primary Visual Cortex
Ke Chen *1,2, Yilei Zhao *1, Ting Liu 1, Zhaohao Su 1, Huiliang Yu 1, Leanne Lai Hang Chan 3,4, Tiejun Liu 1,2, Dezhong Yao 1,2
1The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for NeuroInformation, University of Electronic Science and Technology of China, 2Sichuan Institute for Brain Science and Brain-inspired Intelligence, 3Department of Electronic Engineering, City University of Hong Kong, 4Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong

Here, we present detailed protocols for monocular visual deprivation and ocular dominance plasticity analysis, which are important methods for studying the neural mechanisms of visual plasticity during the critical period and the effects of specific genes on visual development.

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Biology

Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay
Yuping Fan *1,2, Yan Teng *3, Fu tong Liu *4, Fengxia Ma 1,2, Alan Y. Hsu 5, Sizhou Feng 1,2, Hongbo R. Luo 5
1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 2Tianjin Institutes of Health Science, 3Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 4Haihe Laboratory of Cell Ecosystem, Tianjin Medical University, 5Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children's Hospital, Dana-Farber /Harvard Cancer Center

This protocol details the preparation of CLON-G to extend the neutrophil lifespan to greater than 5 days and provides a reliable procedure for evaluating neutrophil death with flow cytometry and confocal fluorescence microscopy.

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Neuroscience

Whole-Mount Immunostaining and Automatic Counting of Mouse Retinal Ganglion Cells
Jialiang Yang *1, Jiaxin Guo *1, Jing Hu *1,2, Xiawei Wu *1, Haotian Huang 1, Yu Wen 1, Runfang Chen 1, Congrui Liu 1, Houbin Zhang 1,3
1Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 2School of Medicine, University of Electronic Science and Technology of China, 3Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

This protocol describes the procedure for isolating the whole-mount mouse retina and performing immunostaining to label all retinal ganglion cells (RGCs). The process is followed by imaging and automatically counting RGCs using AI-based software, providing a simple, fast, and accurate method for quantifying RGCs in the entire mouse retina.

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