Whole-Mount Immunostaining and Automatic Counting of Mouse Retinal Ganglion Cells
809 Views
•
05:52 min
•
October 4th, 2024
DOI :
October 4th, 2024
•Jialiang Yang*1, Jiaxin Guo*1, Jing Hu*1,2, Xiawei Wu*1, Haotian Huang1, Yu Wen1, Runfang Chen1, Congrui Liu1, Houbin Zhang1,3
1Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 2School of Medicine, University of Electronic Science and Technology of China, 3Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
Chapters in this video
0:00
Introduction
1:00
Isolation and Processing of Whole‐Mount Retinas for Immunostaining of Mouse Retinal Ganglion Cells
3:23
Immunostaining and Automated Counting of Mouse Retinal Ganglion Cells Using an AI‐Based Program
Related Videos
11:46
Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
12.3K Views
08:47
Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
45.6K Views
08:54
Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
14.4K Views
11:01
Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry
14.9K Views
08:32
Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
23.0K Views
12:28
Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
3.5K Views
03:47
Methanol-Based Whole-Mount Preparation for the Investigation of Retinal Ganglion Cells
3.2K Views
07:01
Preparation and Analysis of Histological Slides of Rat and Mouse Eyeballs to Evaluate the Retina
700 Views
01:09
Two-Photon In Vivo Imaging of the Mouse Retina
32 Views
01:29
Quantification of Retinal Ganglion Cell Somas Following Cauterization-Induced Ocular Hypertension
26 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved