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Weill Cornell Medicine

5 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
George Zhang 1, Yudan Chi 1, Yi-Chieh Nancy Du 1
1Department of Pathology and Laboratory Medicine, Weill Cornell Medicine

We present a protocol to demonstrate a novel somatic gene transfer system utilizing RIP-Tag; RIP-tva mouse model to study the function of genes in metastasis. The avian retroviruses are delivered intracardiacally to ensure gene transfer into pre-malignant, noninvasive lesions of pancreatic β cells in adult mice.

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Engineering

Rapid Manufacturing of Thin Soft Pneumatic Actuators and Robots
Amir Ali Amiri Moghadam 1,2,3, Alexandre Caprio 1,2, Seyedhamidreza Alaie 1,2, James K. Min 1,2, Simon Dunham 1,2, Bobak Mosadegh 1,2
1Dalio Institute of Cardiovascular Imaging, New York-Presbyterian Hospital, Weill Cornell Medicine, 2Department of Radiology, Weill Cornell Medicine, 3Mechatronics Engineering Department, Kennesaw State University

This protocol describes a method for rapid manufacturing of soft pneumatic actuators and robots with a thin form factor. The fabrication method starts with lamination of thermoplastic polyurethane (TPU) sheets followed by laser cutting/welding of a two-dimensional pattern to form actuators and robots.

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Developmental Biology

Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish
Laila Abd Elmagid *1,2, Nishant Mittal *1,2, Isaac Bakis 1,2, Ching-Ling Lien 3, Michael R. Harrison 1,2
1Cardiovascular Research Institute, Weill Cornell Medicine, 2Department of Cell and Developmental Biology, Weill Cornell Medicine, 3The Saban Research Institute and Heart Institute, Children's Hospital Los Angeles

The present protocol allows efficient and stable delivery of fluorescent microspheres (MS) and quantum dots (QD) into the myocardium of live fish that can be tracked (traced) over time.

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Cellular And Small Animal Models Of Neurodegenerative Diseases
Jacqueline Burré 1, Manu Sharma 1
1Appel Alzheimer’s Disease Research Institute, Brain and Mind Research Institute, Weill Cornell Medicine

Cellular And Small Animal Models Of Neurodegenerative Diseases

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Biology

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Pania E. Bridge-Comer 1, Shannon M. Reilly 1
1Weill Center for Metabolic Health, Department of Medicine, Weill Cornell Medicine

Triglyceride lipolysis in adipocytes is an important metabolic process resulting in the liberation of free fatty acids and glycerol. Here, we provide a detailed protocol to measure basal and stimulated lipolysis in adipocytes and ex vivo adipose tissue from mice.

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