Collection and Concentration of Viruses Expressing Metastatic Factor
2:26
Viral Titer Determination
4:37
Intracardiac Injection
7:24
Results: Detection of Metastatic Pancreatic β-cells by Immunostaining
8:16
Conclusion
Transcript
The overall goal of this procedure is to identify and characterize metastatic factors by gene transfer into the Novel RIP-Tag;RIP-tva mouse model. This method can help answer key questions in the cancer metastasis field such as identifying the dri
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We present a protocol to demonstrate a novel somatic gene transfer system utilizing RIP-Tag; RIP-tva mouse model to study the function of genes in metastasis. The avian retroviruses are delivered intracardiacally to ensure gene transfer into pre-malignant, noninvasive lesions of pancreatic β cells in adult mice.