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University of Hyogo

2 ARTICLES PUBLISHED IN JoVE

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Environment

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
Masaki Miya 1, Toshifumi Minamoto 2, Hiroki Yamanaka 3, Shin-ichiro Oka 4, Keiichi Sato 4, Satoshi Yamamoto 2, Tetsuya Sado 1, Hideyuki Doi 5
1Department of Ecology and Environmental Sciences, Natural History Museum and Institute, Chiba, 2Graduate School of Human Development and Environment, Kobe University, 3Faculty of Science and Technology, Ryukoku University, 4Okinawa Churashima Research Center, 5Graduate School of Simulation Studies, University of Hyogo

We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.

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Environment

Measuring the Flight Ability of the Ambrosia Beetle, Platypus Quercivorus (Murayama), Using a Low-Cost, Small, and Easily Constructed Flight Mill
Ryuichi Okada 1, Duy Long Pham 2, Yasuto Ito 3, Michimasa Yamasaki 2, Hidetoshi Ikeno 1
1School of Human Science and Environment, University of Hyogo, 2Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, 3Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries

We developed a low cost and small flight mill, constructed with commonly available items and easily used in experimentation. Using this apparatus, we measured the flight ability of an ambrosia beetle, Platypus quercivorus.

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