Filtration of Water Samples ≤4 L Using the Filter Cartridge
3:05
Filtration of Water Samples >4 L Using the Filter Cartridge
4:23
Extraction of eDNA from the Filter
6:57
Results: MiFish Metabarcoding of eDNA
7:23
Conclusion
Transcript
The overall goal of this protocol is to filter water samples with a filter cartridge and extract environmental DNA without having to cut open the housing to remove the filter from metabarcoding eDNA from various aquatic organisms. This method can
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We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.