University of Hyogo

2 ARTICLES PUBLISHED IN JoVE

Environment

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Masaki Miya¹, Toshifumi Minamoto², Hiroki Yamanaka³, Shin-ichiro Oka⁴, Keiichi Sato⁴, Satoshi Yamamoto², Tetsuya Sado¹, Hideyuki Doi⁵

¹Department of Ecology and Environmental Sciences, Natural History Museum and Institute, Chiba, ²Graduate School of Human Development and Environment, Kobe University, ³Faculty of Science and Technology, Ryukoku University, ⁴Okinawa Churashima Research Center, ⁵Graduate School of Simulation Studies, University of Hyogo

We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.

Environment

Measuring the Flight Ability of the Ambrosia Beetle, Platypus Quercivorus (Murayama), Using a Low-Cost, Small, and Easily Constructed Flight Mill

Ryuichi Okada¹, Duy Long Pham², Yasuto Ito³, Michimasa Yamasaki², Hidetoshi Ikeno¹

¹School of Human Science and Environment, University of Hyogo, ²Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, ³Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries

We developed a low cost and small flight mill, constructed with commonly available items and easily used in experimentation. Using this apparatus, we measured the flight ability of an ambrosia beetle, Platypus quercivorus.