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Federal Environment Agency

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
Katrin Süring 1, Sabine Bach 1, Conny Höflich 1, Wolfgang Straff 1
1Environmental Medicine and Health Effects Assessment, Federal Environment Agency

Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.

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Biology

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Meike Koester 1,2, René Gergs 1,3
1Institute for Environmental Sciences, University of Koblenz-Landau, 2Institute of Integrated Natural Sciences, University of Koblenz-Landau, 3IV 2.5 (Trace Analysis, Artificial Pond and stream system), Federal Environment Agency

Most common gut content analyses of macroinvertebrates are visual. Requiring intense knowledge about morphological diversity of prey organisms, they miss soft bodied prey and, due to strong comminution of prey, are nearly impossible for some organisms, including amphipods. We provide detailed, novel genetic approaches for macroinvertebrate prey identification in the diet of amphipods.

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