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Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

DOI :

10.3791/54721-v

10:42 min

November 19th, 2016

November 19th, 2016

7,097 Views

1Environmental Medicine and Health Effects Assessment, Federal Environment Agency

Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.

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Flow Cytometric Analysis

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