JoVE Logo
Faculty Resource Center

Sign In

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

DOI :

10.3791/54721-v

10:42 min

November 19th, 2016

November 19th, 2016

7,133 Views

1Environmental Medicine and Health Effects Assessment, Federal Environment Agency

Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.

Tags

Flow Cytometric Analysis

-- Views

Related Videos

article

Murine Model of Allergen Induced Asthma

article

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

article

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

article

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

article

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

article

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

article

Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

article

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

article

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

article

Multi-scale Analysis of Bacterial Growth Under Stress Treatments

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved