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Ariel University

7 ARTICLES PUBLISHED IN JoVE

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Education

Exploring the Radical Nature of a Carbon Surface by Electron Paramagnetic Resonance and a Calibrated Gas Flow
Uri Green 1,2, Yulia Shenberger 3, Zeev Aizenshtat 1, Haim Cohen 2,4, Sharon Ruthstein 3
1Chemistry Institute, The Hebrew University of Jerusalem, 2Biological Chemistry Department, Ariel University, 3Chemistry Department, Faculty of Exact Science, Bar Ilan University, 4Chemistry Department, Ben-Gurion University

Stable radicals that are present in carbon substrates interact with paramagnetic oxygen through a Heisenberg spin exchange. This interaction can be significantly reduced under STP conditions by flowing a diamagnetic gas over the carbon system. This manuscript describes a simple method to characterize the nature of those radicals.

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Behavior

The Emotional Stroop Task: Assessing Cognitive Performance under Exposure to Emotional Content
Moshe Shay Ben-Haim 1, Paul Williams 2, Zachary Howard 2, Yaniv Mama 3, Ami Eidels 2, Daniel Algom 1
1School of Psychological Sciences, Tel-Aviv University, 2School of Psychology, University of Newcastle, 3Department of Behavioral Sciences, Ariel University

The emotional Stroop effect (ESE) is the result of longer naming latencies to ink colors of emotion words than those of neutral words. This report refers to potential sources of confounding and includes a modal experiment that provides the means to control for them.

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Biology

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Ayelet Di Segni 1, Tzipi Braun 1, Marina BenShoshan 1, Sarit Farage Barhom 1, Efrat Glick Saar 1, Karen Cesarkas 1, James E. Squires 2, Nathan Keller 1,3, Yael Haberman 1,4
1Sheba Medical Center, 2Children's Hospital of Pittsburgh of UPMC, 3Ariel University, 4Cincinnati Children's Hospital Medical Center

This manuscript describes a detailed standardized protocol of high-throughput 16S rRNA-amplicon sequencing. The protocol introduces an integrated, uniformed, feasible, and inexpensive protocol starting from fecal sample collection through data analyses. This protocol enables analysis of large numbers of samples with rigorous standards and several controls.

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JoVE Core

A Two-Step Protocol for Umpolung Functionalization of Ketones Via Enolonium Species
Shlomy Arava 1, Shimon Maksymenko 1, Keshaba Nanda Parida 1, Gulab K. Pathe 1, Atul M. More 1, Yuriy B. Lipisa 1, Alex M. Szpilman 1
1Department of Chemical Sciences, Ariel University

A two step one-pot protocol for the umpolung of ketone enolates to enolonium species and addition of a nucleophile to the α-position is described. Nucleophiles include chloride, azide, azoles, allyl-silanes, and aromatic compounds.

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Bioengineering

Increasing Durability of Dissociated Neural Cell Cultures Using Biologically Active Coralline Matrix
Orly Eva Weiss 1, Roni Mina Hendler 1, Danny Baranes 1
1Department of Molecular Biology, Ariel University

Dissociated hippocampal cell culture is a pivotal experimental tool in neuroscience. Neural cell survival and function in culture is enhanced when coralline skeletons are used as matrices, due to their neuroprotective and neuromodulative roles. Hence, neural cells grown on coralline matrix show higher durability, and thereby are more adequate for culturing.

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Neuroscience

Automatic Identification of Dendritic Branches and their Orientation
Inbar Dahari 1, Danny Baranes 1, Refael Minnes 2
1Department of Molecular Biology, Ariel University, 2Department of Physics, Ariel University

Presented is a computational tool that allows simple and direct automatic measurement of orientations of neuronal dendritic branches from 2D fluorescence images.

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Neuroscience

New Framework for Understanding Cross-Brain Coherence in Functional Near-Infrared Spectroscopy (fNIRS) Hyperscanning Studies
Hila Zahava Gvirts Provolovski 1, Mini Sharma 1, Itai Gutman 1, Anat Dahan 2, Yafeng Pan 3, Jacqueline Stotler 4, Teresa Wilcox 4
1The Department of Behavioral Sciences and Psychology, Ariel University, 2Braude College of Engineering, 3Department of Psychology and Behavioral Sciences, Zhejiang University, 4Department of Psychology, Florida Atlantic University

Wavelet transform coherence (WTC) is a common methodology for assessing the coupling between signals that is used in functional near-infrared spectroscopy (fNIRS) hyperscanning studies. A toolbox for assessing the directionality of the signal interaction is presented in this work.

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