Ariel University

7 ARTICLES PUBLISHED IN JoVE

Education

Exploring the Radical Nature of a Carbon Surface by Electron Paramagnetic Resonance and a Calibrated Gas Flow

Uri Green^1,2, Yulia Shenberger³, Zeev Aizenshtat¹, Haim Cohen^2,4, Sharon Ruthstein³

¹Chemistry Institute, The Hebrew University of Jerusalem, ²Biological Chemistry Department, Ariel University, ³Chemistry Department, Faculty of Exact Science, Bar Ilan University, ⁴Chemistry Department, Ben-Gurion University

Stable radicals that are present in carbon substrates interact with paramagnetic oxygen through a Heisenberg spin exchange. This interaction can be significantly reduced under STP conditions by flowing a diamagnetic gas over the carbon system. This manuscript describes a simple method to characterize the nature of those radicals.

Behavior

The Emotional Stroop Task: Assessing Cognitive Performance under Exposure to Emotional Content

Moshe Shay Ben-Haim¹, Paul Williams², Zachary Howard², Yaniv Mama³, Ami Eidels², Daniel Algom¹

¹School of Psychological Sciences, Tel-Aviv University, ²School of Psychology, University of Newcastle, ³Department of Behavioral Sciences, Ariel University

The emotional Stroop effect (ESE) is the result of longer naming latencies to ink colors of emotion words than those of neutral words. This report refers to potential sources of confounding and includes a modal experiment that provides the means to control for them.

Biology

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Ayelet Di Segni¹, Tzipi Braun¹, Marina BenShoshan¹, Sarit Farage Barhom¹, Efrat Glick Saar¹, Karen Cesarkas¹, James E. Squires², Nathan Keller^1,3, Yael Haberman^1,4

¹Sheba Medical Center, ²Children's Hospital of Pittsburgh of UPMC, ³Ariel University, ⁴Cincinnati Children's Hospital Medical Center

This manuscript describes a detailed standardized protocol of high-throughput 16S rRNA-amplicon sequencing. The protocol introduces an integrated, uniformed, feasible, and inexpensive protocol starting from fecal sample collection through data analyses. This protocol enables analysis of large numbers of samples with rigorous standards and several controls.

JoVE Core

A Two-Step Protocol for Umpolung Functionalization of Ketones Via Enolonium Species

Shlomy Arava¹, Shimon Maksymenko¹, Keshaba Nanda Parida¹, Gulab K. Pathe¹, Atul M. More¹, Yuriy B. Lipisa¹, Alex M. Szpilman¹

¹Department of Chemical Sciences, Ariel University

A two step one-pot protocol for the umpolung of ketone enolates to enolonium species and addition of a nucleophile to the α-position is described. Nucleophiles include chloride, azide, azoles, allyl-silanes, and aromatic compounds.

Bioengineering

Increasing Durability of Dissociated Neural Cell Cultures Using Biologically Active Coralline Matrix

Orly Eva Weiss¹, Roni Mina Hendler¹, Danny Baranes¹

¹Department of Molecular Biology, Ariel University

Dissociated hippocampal cell culture is a pivotal experimental tool in neuroscience. Neural cell survival and function in culture is enhanced when coralline skeletons are used as matrices, due to their neuroprotective and neuromodulative roles. Hence, neural cells grown on coralline matrix show higher durability, and thereby are more adequate for culturing.

Neuroscience

Automatic Identification of Dendritic Branches and their Orientation

Inbar Dahari¹, Danny Baranes¹, Refael Minnes²

¹Department of Molecular Biology, Ariel University, ²Department of Physics, Ariel University

Presented is a computational tool that allows simple and direct automatic measurement of orientations of neuronal dendritic branches from 2D fluorescence images.

Neuroscience

New Framework for Understanding Cross-Brain Coherence in Functional Near-Infrared Spectroscopy (fNIRS) Hyperscanning Studies

Hila Zahava Gvirts Provolovski¹, Mini Sharma¹, Itai Gutman¹, Anat Dahan², Yafeng Pan³, Jacqueline Stotler⁴, Teresa Wilcox⁴

¹The Department of Behavioral Sciences and Psychology, Ariel University, ²Braude College of Engineering, ³Department of Psychology and Behavioral Sciences, Zhejiang University, ⁴Department of Psychology, Florida Atlantic University

Wavelet transform coherence (WTC) is a common methodology for assessing the coupling between signals that is used in functional near-infrared spectroscopy (fNIRS) hyperscanning studies. A toolbox for assessing the directionality of the signal interaction is presented in this work.