Neural Stem Cell Institute

2 ARTICLES PUBLISHED IN JoVE

Medicine

Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye

Katherine J. Wert^1,2, Jessica M. Skeie^3,4, Richard J. Davis¹, Stephen H. Tsang^1,3, Vinit B. Mahajan^3,4

¹Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, Columbia University , ²Institute of Human Nutrition, College of Physicians & Surgeons, Columbia University , ³Omics Laboratory, University of Iowa , ⁴Department of Ophthalmology and Visual Sciences, University of Iowa

This surgical technique illustrates the injection of gene therapy vectors and stem cells into the subretinal space of the mouse eye.

Neuroscience

Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes

Cuiping Zhao¹, Nathan C. Boles¹, Justine D. Miller¹, Suzanne Kawola¹, Sally Temple¹, Richard J. Davis^*1, Jeffrey H. Stern^*1

¹Neural Stem Cell Institute

Subretinal injection has been widely applied in preclinical studies of stem cell replacement therapy for age-related macular degeneration. In this visualized article, we describe a less risky, reproducible and precisely modified subretinal injection technique via the trans-scleral approach to deliver cells into rat eyes.