The overall goal of this video is to introduce a reproducible trans-scleral subretinal injection technique to deliver human retinal pigment epithelial, or RPE cells, into the subretinal space of rodent's eyes with minimal risk of retina damage. Th
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Subretinal injection has been widely applied in preclinical studies of stem cell replacement therapy for age-related macular degeneration. In this visualized article, we describe a less risky, reproducible and precisely modified subretinal injection technique via the trans-scleral approach to deliver cells into rat eyes.