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Eye and ENT Hospital of Fudan University

1 ARTICLES PUBLISHED IN JoVE

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Biology

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
Fengxi Meng *1,2, Xiaofeng Li *1,2, Hui Ren 1,2, Jiang Qian 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University

Small ubiquitin-related modifier (SUMO) family proteins are conjugated to the lysine residues of target proteins to regulate various cellular processes. This paper describes a protocol for the detection of retinoblastoma (Rb) protein SUMOylation under endogenous and exogenous conditions in human cells.

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