In Vivo Detection and Analysis of Rb Protein SUMOylation in Human CellsFengxi Meng *1,2, Xiaofeng Li *1,2, Hui Ren 1,2, Jiang Qian 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University
Small ubiquitin-related modifier (SUMO) family proteins are conjugated to the lysine residues of target proteins to regulate various cellular processes. This paper describes a protocol for the detection of retinoblastoma (Rb) protein SUMOylation under endogenous and exogenous conditions in human cells.
