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Skolkovo Institute of Science and Technology

3 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Imaging of Extracellular Vesicles by Atomic Force Microscopy
Mikhail Skliar 1,2, Vasiliy S. Chernyshev 3,4
1Department of Chemical Engineering, University of Utah, 2The Nano Institute of Utah, University of Utah, 3Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster 'Northern', Moscow Institute of Physics and Technology

A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties. 

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Developmental Biology

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
Rita Silvério-Alves 1,2,3, Andreia M. Gomes 3, Ilia Kurochkin 4, Kateri A. Moore 5,6, Carlos-Filipe Pereira 1,2,3
1Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 2Wallenberg Center for Molecular, Lund University, 3Center for Neuroscience and Cell Biology, University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai

This protocol demonstrates the induction of a hemogenic program in human dermal fibroblasts by enforced expression of the transcription factors GATA2, GFI1B and FOS to generate hematopoietic stem and progenitor cells.

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Engineering

Membraneless Hydrogen Peroxide Fuel Cells as a Promising Clean Energy Source
Fenyang Zhu 1, Guoxiang Chen 1, Aleksei Kuzin 1,2, Dmitry A. Gorin 2, Brij Mohan 3, Gaoshan Huang 1, Yongfeng Mei 1,4,5,6,7, Alexander A. Solovev 1
1Department of Materials Science & State Key Laboratory of Molecular Engineering of Polymers, Fudan University, 2Center for Photonic Science and Engineering, Skolkovo Institute of Science and Technology, 3Centro de Quimica Estrutural, Institute of Molecular Sciences, Instituto Superior Tecnico, Universidade de Lisboa, 4Center for Biomedical Engineering, School of Information Science and Technology, Fudan University, 5International Institute of Intelligent Nanorobots and Nanosystems, Fudan University, 6Shanghai Frontiers Science Research Base of Intelligent Optoelectronics and Perception, Fudan University, 7Yiwu Research Institute of Fudan University

This protocol introduces the design and evaluation of innovative three-dimensional electrodes for hydrogen peroxide fuel cells, utilizing Au-electroplated carbon fiber cloth and Ni-foam electrodes. The research findings highlight hydrogen peroxide's potential as a promising candidate for sustainable energy technologies.

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