JoVE Logo

Sign In

Fujian Medical University

11 ARTICLES PUBLISHED IN JoVE

image

Biology

Visualization of the Interstitial Cells of Cajal (ICC) Network in Mice
Yu Chen 1,2, Tambudzai Shamu 2, Hui Chen 3, Peter Besmer 3, Charles L. Sawyers 2,4, Ping Chi 1,5
1Department of Medicine, Memorial Sloan Kettering Cancer Center, 2Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, 3Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 4Howard Hughes, Medical Institute, 5Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University

The interstitial cells of Cajal (ICC) are the pacemaker cells of the gastrointestinal (GI) tract. They form complex networks between smooth muscle cells and post-ganglionic neuronal fibers to regulate GI contractility. Here, we present immunofluorescence methods cross-sectional and whole-mount visualization of murine ICC networks.

image

Medicine

A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects
Liang Feng 1,2, Hui Chen 1, Genn Suyeoka 1, Xiaorong Liu 1,2
1Department of Ophthalmology, Northwestern University, 2Department of Neurobiology, Northwestern University

Chronic ocular hypertension is induced using laser photocoagulation of the trabecular meshwork in mouse eyes. The intraocular pressure (IOP) is elevated for several months after laser treatment. The decrease of visual acuity and contrast sensitivity of experimental animals are monitored using the optomotor test.

image

Behavior

Using Laser Doppler Imaging and Monitoring to Analyze Spinal Cord Microcirculation in Rat
Yingli Jing *1,2,3,4, Fan Bai *1,2,3,4, Hui Chen 1,2,3,4, Hao Dong 1,2,3,4
1China Rehabilitation Research Center, 2Institute of Rehabilitation Science of China, 3Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, 4Beijing Key Laboratory of Neural Injury and Rehabilitation

Here we present a combination of laser Doppler perfusion imaging (LDPI) and laser Doppler perfusion monitoring (LDPM) to measure spinal cord local blood flows and oxygen saturation (SO2), as well as a standardized procedure for introducing spinal cord trauma on rat.

image

Cancer Research

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
Xiaoxing Lv 1, Meiru Zhao 1, Yuting Yi 1, Lucheng Zhang 1, Yanfang Guan 1, Tao Liu 1, Ling Yang 1, Rongrong Chen 1, Jianhui Ma 1, Xin Yi 1
1Geneplus-Beijing Institute

This manuscript describes a technique for detecting mutations of low frequency in ctDNA, ER-Seq. This method is differentiated by its unique use of two-directional error correction, a special background filter, and efficient molecular acquirement.

image

Neuroscience

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
Mengye Zhu 1, Daying Zhang 1, Sicong Peng 2, Nana Liu 2, Jing Wu 2, Haixia Kuang 2, Tao Liu 2,3
1Department of Pain, First Affiliated Hospital of Nanchang University, 2Department of Pediatrics, First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University

Here, we describe the essential steps for whole-cell patch-clamp recordings made from substantia gelatinosa (SG) neurons in the in vitro spinal cord slice. This method allows the intrinsic membrane properties, synaptic transmission and morphological characterization of SG neurons to be studied.

image

Medicine

OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery
Tao Liu *1,2,3,4,5, Xueling Song *1,2,3,4,5, Xiaoying Zheng *1,2,3,4,5, Liying Yan 1,2,3,4,5, Caihong Ma 1,2,3,5, Rong Li 1,2,3,4,5, Jie Yan 1,2,3,4,5, Jie Qiao 1,2,3,4,5
1Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology, 3Key Laboratory of Assisted Reproduction, Ministry of Education, 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, 5Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Chinese Academy of Medical Sciences

In vitro maturation (IVM) before gynecological operation (OP-IVM) combines IVM following oocyte retrieval with routine gynecological surgery and serves as an extension of conventional IVM applications for fertility preservation.

image

Biology

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
Meng-Fei Xu 1,2, Yu-Hao Yang 1,2, Ya-Lan Wei 3,4, Jing-Lian Zhang 1,2, Xuan Lin 1,2, Xiao-Yan Lin 1,2, Hui Chen 1,2, Zhen-Yu She 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University

This article reports an in vivo inhibition of CENP-E through abdominal surgery and testicular injection of GSK923295, a valuable model for male meiotic division. Using the immunofluorescence, flow cytometry and transmission electron microscopy assays, we show that CENP-E inhibition results in chromosome misalignment and genome instability in mouse spermatocytes.

image

Behavior

Usability Evaluation of Augmented Reality: A Neuro-Information-Systems Study
Jun Wu *1, Di Zhang *1, Tao Liu 2,3,4, Helen Hong Yang 5, Yi Wang 1, Huili Yao 1, Shinan Zhao 1
1Economics and Management School, Jiangsu University of Science and Technology, 2School of Health, Fujian Medical University, 3School of Management, Shanghai University, 4School of Management, Zhejiang University, 5Business School, La Trobe University

This study presents an experimental paradigm for a usability test combining subjective and objective evaluations. The objective evaluation adopted Neuro-Information-Systems (NeuroIS) methods, and the subjective evaluation adopted a usability questionnaire and a NASA-Task Load Index (NASA-TLX) scale.

image

Medicine

Plasma Polishing as a New Polishing Option to Reduce the Surface Roughness of Porous Titanium Alloy for 3D Printing
Zhiwei Lin *1,2, Lincong Luo *3, Dongxin Lin *4, Yuping Deng 4,5, Yang Yang 4, Xuecheng Huang 6, Tingrui Wu 1, Wenhua Huang 1,2,4
1Orthopedic Center, Affiliated Hospital of Guangdong Medical University, 2The School of Basic Medical Sciences, Guangdong Medical University, 3The School of Basic Medical Sciences, Fujian Medical University, 4Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Digital Medicine and Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, 5Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, 6Shenzhen Hospital of Guangzhou University of Chinese Medicine (Futian)

Plasma polishing is a promising surface processing technology, especially suitable for 3D printing of porous titanium alloy workpieces. It can remove semi-molten powders and ablative oxide layers, thereby effectively reducing surface roughness and improving surface quality.

image

Biology

Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System
Meng-Fei Xu 1,2, Jie Chen 1,2, Yue Xu 1,2, Jing-Lian Zhang 1,2, Yi Zhou 1,2, Jie-Jie He 1,2, Shan Wu 1,2, Ya-Lan Wei 3,4, Zhen-Yu She 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 4Medical Research Center, Fujian Maternity and Child Health Hospital

This article reports the construction of centromere-associated protein-E (CENP-E) knockout cells using the CRISPR/Cas9 system and three phenotype-based screening strategies. We have utilized the CENP-E knockout cell line to establish a novel approach to validate the specificity and toxicity of the CENP-E inhibitors, which is useful for drug development and biological research.

image

Biology

Epon Post Embedding Correlative Light and Electron Microscopy
Shuyuan Wang *1, Haiyan Xiong *1,2, Qiyuan Chang *1,3, Xudong Zhuang *4,5,6, Yaochen Wu 1,3, Xinrui Wang 4,5,6, Congxian Wu 1, Zhifei Fu 1,7,8
1Public Technology Service Center, Fujian Medical University, 2The School of Pharmacy, Fujian Medical University, 3The School of Basic Medical Sciences, Fujian Medical University, 4College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 5Medical Research Center, Fujian Maternity and Child Health Hospital, 6NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, Fujian Maternity and Child Health Hospital, 7Institute of Neuroscience, Fujian Medical University, 8Fujian Key Laboratory of Molecular Neurology, Fujian Medical University

We present a detailed protocol for Epon post-embedding correlative light and electron microscopy using a fluorescent protein called mScarlet. This method can maintain the fluorescence and the ultrastructure simultaneously. This technique is amenable to a wide variety of biological applications.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved