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Medical University of Warsaw

4 ARTICLES PUBLISHED IN JoVE

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Medicine

An In Vivo Method for Evaluating the Gut-Blood Barrier and Liver Metabolism of Microbiota Products
Kinga Jaworska 1, Tomasz Huc 1, Marta Gawrys 1, Maksymilian Onyszkiewicz 1, Emilia Samborowska 2, Marcin Ufnal 1
1Department of Experimental Physiology and Pathophysiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, 2Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences

The access of nutrients, microbiota metabolites and medicines to the circulation is controlled by the gut-blood barrier (GBB). We describe a direct method for measuring the GBB permeability in vivo, which, in contrast to commonly used indirect methods, is virtually not affected by liver and kidney functions.

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Medicine

The Use of Mixed Reality in Custom-Made Revision Hip Arthroplasty: A First Case Report
Paweł Łęgosz 1, Krzysztof Starszak 1,2, Maciej Stanuch 3,4, Maciej Otworowski 1, Łukasz Pulik 1, Adriana Złahoda-Huzior 3,4, Andrzej Skalski 3,4
1Department of Orthopaedics and Traumatology of the Musculoskeletal System, Infant Jesus Teaching Hospital, Medical University of Warsaw, 2Department of Human Anatomy, Medical University of Silesia, 3Department of Measurement and Electronics, AGH University of Science and Technology, 4MedApp S.A.

A complex revision hip arthroplasty was performed using a custom-made implant and mixed reality technology. According to the authors' knowledge, this is the first report of such procedure described in the literature.

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Bioengineering

Building Up Skin Models for Numerous Applications - from Two-Dimensional (2D) Monoculture to Three-Dimensional (3D) Multiculture
Adrianna Maria Piasek *1, Iryna Levkovych *1, Paulina Musolf 1, Hanna Chmielewska 1, Aneta Ścieżyńska 2, Anna Sobiepanek 1
1Chair of Drug and Cosmetics Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 2Department of Histology and Embryology, Medical University of Warsaw

Here, we present inexpensive and simple procedures to introduce various 3D skin models for routine research in a cell culture laboratory. Researchers can create models tailored to their needs without relying on commercially available models.

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Neuroscience

Motion-Acuity Test for Visual Field Acuity Measurement with Motion-Defined Shapes
Marco Ninghetto 1, Michał Wieteska 2,3, Anna Kozak 1, Kamil Szulborski 4, Tomasz Gałecki 4, Jacek Szaflik 4, Kalina Burnat 1
1Nencki Institute of Experimental Biology, Polish Academy of Sciences, 2Mossakowski Medical Research Institute, Polish Academy of Sciences, 3Institute of Radio Electronics and Multimedia Technology, Warsaw University of Technology, 4Department of Ophthalmology, Medical University of Warsaw

A novel motion-based acuity test that allows the assessment of central and peripheral visual processing in low-vision and healthy individuals, along with goggles limiting peripheral vision compatible with MRI protocols, are described here. This method offers a comprehensive vision assessment for functional impairments and dysfunctions of the visual system.

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