A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMCGabriele Babini *1, Jacopo Morini *1, Sofia Barbieri 1, Giorgio Baiocco 1, Giovanni Battista Ivaldi 2, Marco Liotta 2, Paola Tabarelli de Fatis 2, Andrea Ottolenghi 1
1Dipartimento di Fisica, Università degli Studi di Pavia, 2IRCCS Istituti Clinici Scientifici Maugeri
We present a protocol to investigate the crosstalk between X-ray-irradiated Caco-2 and peripheral blood mononuclear cells (PBMC). The protocol starts with Caco-2 irradiation and set-up of the co-culture with PBMC; subsequently, trans-epithelial electrical resistance is measured regularly over 48 h and western blot performed in both Caco-2 and PBMC.
