A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
12.9K Views
•
11:40 min
•
January 30th, 2018
DOI :
January 30th, 2018
•Chapters in this video
0:04
Title
1:05
Cell Preparation and Irradiation
3:21
Cell Metabolic Activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT]) and Viability Assays, and Trans-Epithelial Electrical Resistance (TEER)
6:12
Western Blot Analysis
9:15
Results: Representative Effects of Caco-2 Cell Radiation Exposure and PBMC Co-culture
11:01
Conclusion
Related Videos
Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
22.1K Views
Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice
16.7K Views
The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis
31.8K Views
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
11.8K Views
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
8.6K Views
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
8.8K Views
Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens
11.3K Views
Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
15.7K Views
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
7.1K Views
Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease
7.7K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved