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Beijing University of Chinese Medicine

25 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Generation of Monoclonal Antibodies Against Natural Products
Yue Zhang 1, Peng Cao 2, Fang Lu 3, Xin Yan 4, Bingqian Jiang 3, Jinjun Cheng 3, Huihua Qu 5
1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3School of Basic Medical Sciences, Beijing University of Chinese Medicine, 4School of Chinese Materia Medica, Beijing University of Chinese Medicine, 5Center of Scientific Experiment, Beijing University of Chinese Medicine

This article provides a detailed protocol for the preparation and evaluation of monoclonal antibodies against natural products for use in various immunoassays. This procedure includes immunization, cell fusion, indirect competitive ELISA for positive clone screening, and monoclonal hybridoma preparation. The specifications for antibody characterization using MALDI-TOF-MS and ELISA analyses are also provided.

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Neuroscience

A Rat Model of Central Fatigue Using a Modified Multiple Platform Method
Weiyue Zhang *1, Wei Zhang *1, Ning Dai *1, Chenxia Han 2, Fengzhi Wu 1, Xu Wang 1, Libo Tan 1, Jie Li 1, Feng Li 1, Qingjia Ren 3
1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 2Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, 3Institute of Tibetan Medicine, Tibetan Traditional Medical College

Here, we present a protocol to introduce a rat model of central fatigue using the modified multiple platform method (MMPM).

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Medicine

A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish
Xiao Ma *1,2,3, Yonghe Ding *2,3, Yong Wang 2,3,4, Xiaolei Xu 1,2,3
1Clinical and Translational Sciences Track, Mayo Clinic Graduate School of Biomedical Sciences, 2Department of Biochemistry and Molecular Biology, Mayo Clinic, 3Division of Cardiovascular Diseases, Mayo Clinic, 4Institute of Life Science, Beijing University of Chinese Medicine

A method to generate a doxorubicin-induced cardiomyopathy model in adult zebrafish (Danio rerio) is described here. Two alternative ways of intraperitoneal injection are presented and conditions to reduce variations among different experimental groups are discussed.

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JoVE Core

Evaluating the Anti-depression Effect of Xiaoyaosan on Chronically-stressed Mice
Zhi-Yi Yan *1, Xiao-Juan Li *1,2, Xiu-Fang Ding 1, Yue-Yun Liu 1, Jia-xu Chen 1,2
1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 2Formula-pattern Research Center, School of Traditional Chinese Medicine, Jinan University

Here, we present a protocol to establish the mouse depressive model, observe the behavior changes associated with chronic unpredictable mild stress (CUMS), and evaluate the anti-depression effect of Xiaoyaosan.

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Behavior

Analysis of Learning and Memory Ability in an Alzheimer's Disease Mouse Model using the Morris Water Maze
Huiling Tian 1, Ning Ding 2, Mengwei Guo 1, Shun Wang 1, Zidong Wang 1, Hao Liu 1, Jiayi Yang 1, Yujie Li 3, Jingyu Ren 1, Jing Jiang 4, Zhigang Li 1
1School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, 2Guang'anmen Hospital of China Academy of Chinese Medical Science, 3The Third Affiliated Hospital of Beijng University of Chinese Medicine, 4School of Nursing, Beijing University of Chinese Medicine

Herein, a protocol to conduct the Morris water maze tests to evaluate the ability of learning and memory of Alzheimer’s Disease model mice and to assess the effect of manual acupuncture for treating them is described.

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Biology

Detecting Migration and Infiltration of Neutrophils in Mice
Qingyi Lu *1, Kai Yuan *1, Xiaohong Li 1, Haixu Jiang 1, Guiyang Huo 1, Wenrui Jia 1, Guangrui Huang 1, Anlong Xu 1,2
1School of Life Sciences, Beijing University of Chinese Medicine, 2State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University

Here, we present three methods to assess neutrophil migration and infiltration both in vivo and in vitro. These methods can be used to discover promising therapeutics targeting neutrophil migration.

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Genetics

Gene Mining and Sequence Analysis of Purine Nucleosidase Based on RNA-Seq
Fenfang Wu 1, Shiping Hu 2, Yun Ran 2, Xiaoni Chen 1, Shan Lin 1
1Department of Central Laboratory, Shenzhen Hospital, Beijing University of Chinese Medicine, 2Department of Hepatopathy, Shenzhen Hospital, Beijing University of Chinese Medicine

In this protocol, a method for gene mining and sequence analysis of purine nucleosidase (PN, EC:3.2.2.1) based on RNA-Seq was described. ProtProm analysis was applied to show the unique secondary and tertiary structures of PN. Furthermore, the PN gene was cloned from transcriptome to verify the reliability of RNA-Seq results.

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Biochemistry

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Yue Zhang *1, Peng Cao *2, Fang Lu 1, Jinjun Cheng 3, Huihua Qu 4
1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, 4Center of Scientific Experiment, Beijing University of Chinese Medicine

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

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Biology

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization
Dingyun Song 1,2, Yicong Chen 1,3, Xin Wang 1,4, Xueying Chen 1,2, Shuwei Gao 1,3, Wenxiu Xu 1,3, Shengnan Yang 5, Zai Wang 6, Liang Peng 7, Huaping Dai 1,2
1Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, National Clinical Research Center for Respiratory Diseases, Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, 2Graduate School of Peking Union Medical College, Chinese Academy of Medical Science and Peking Union Medical College, 3Capital Medical University, 4Beijing University of Chinese Medicine, 5Tianjin Chest Hospital, 6Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, 7Beijing Key Laboratory for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital

Various animal models of pulmonary fibrosis have been established using bleomycin to clarify the pathogenesis of pulmonary fibrosis and find new drug targets. However, most pulmonary fibrosis models targeting lung tissue have uneven drug administration. Here, we propose a model of uniform pulmonary fibrosis induced by nasal bleomycin nebulization.

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Medicine

3D Printing Model of a Patient's Specific Lumbar Vertebra
Huizhong Bai 1, Zhuoluo Zhou 1, Gang Liu 1, Shengyuan Jiang 1, Yaqi Zhang 1, Xinwei Zuo 1, Fangliang Xing 2, Lin Xu 1, Li Wang 3, Xiaohong Mu 1
1Department of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, 2Beijing Intelligent Entropy Science & Technology Co Ltd., 3Beijing An Yuan Quan Lv Medical Research Institute

This study aims to create a 3D-printed model of a patient-specific lumbar vertebra, which contains both the vertebra and spinal nerve models fused from high-resolution computed tomography (HRCT) and MRI-Dixon data.

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Medicine

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
Jie Chen 1, Yu-fei Li 1, Yun-shu Zhang 1, Tian-hui Du 1, Yang Lu 1, Xin-yi Li 1, Shu-wen Guo 1,2
1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 2Fangshan Chinese Medicine Hospital, Beijing University of Chinese Medicine

Based on in vitro experiments, this study revealed the mechanism of crocetin in repairing oxidative stress damage of cardiomyocytes by influencing mitophagy, in which the PINK1/Parkin signaling pathway plays an important role.

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Medicine

Fu's Subcutaneous Needling for Knee Osteoarthritis Pain
Po-En Chiu *1,2, Zhonghua Fu *3,4, Jian Sun 4,5,6, Guan-Wei Jian 7,8, Te-Mao Li 9, Li-Wei Chou 10,11,12
1Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, 2Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, 3Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, 4Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, 5Second Clinical Medical College, Guangzhou University of Chinese Medicine, 6Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 7Graduate Institute of Acupuncture Science, China Medical University, 8Department of Chinese Medicine, Sinying Hospital, Ministry of Health and Welfare, 9School of Chinese Medicine, College of Chinese Medicine, China Medical University, 10Department of Physical Medicine and Rehabilitation, China Medical University Hospital, 11Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, 12Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University

We present a protocol for using Fu's subcutaneous needling for knee osteoarthritis pain, which combines swaying movement and reperfusion approach techniques. This protocol has great potential for future applications in myofascial pain treatment and could enhance Fu's subcutaneous needling (FSN) manipulation skills.

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Medicine

Efficacy of Fu's Subcutaneous Needling on Sciatic Nerve Pain: Behavioral and Electrophysiological Changes in a Chronic Constriction Injury Rat Model
Po-En Chiu *1,2, Zhonghua Fu *3,4, De-Wei Lai 5,6, Li-Wei Chou 7,8,9
1Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, 2Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, 3Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, 4Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, 5Experimental Animal Center, Department of Molecular Biology and Cell Research, Chang Bing Show Chwan Memorial Hospital, 6Department of Pharmacy and Master Program, Tajen University, 7Department of Physical Medicine and Rehabilitation, China Medical University Hospital, 8Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, 9Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University

We present a protocol for using Fu's subcutaneous needling in a chronic constriction injury model to induce sciatic nerve pain in rats.

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Medicine

A 3D Digital Model for the Diagnosis and Treatment of Pulmonary Nodules
Jingwen Xue 1, Fangliang Xing 2, Yang Liu 3, Tengxiao Liang 4
1Beijing University of Chinese Medicine, 2Beijing Intelligent Entropy Science & Technology Co Ltd., 3Center for Integrated Chinese and Western Medicine, Beijing You An Hospital, Capital Medical University, 4Fever Outpatient Clinic, Dongzhimen Hospital of Beijing University of Chinese Medicine

The objective of this study is to develop a novel 3D digital model of pulmonary nodules that serves as a communication bridge between physicians and patients and is also a cutting-edge tool for pre-diagnosis and prognostic evaluation.

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Medicine

Application of Acupotomy in a Knee Osteoarthritis Model in Rabbit
Xing LongFei 1, Guo Yan 2, Chen XiLin 1, Hu TingYao 1, Zhu WenTing 3, Ma WeiWei 1, Du Mei 1, Xu Yue 1, Guo ChangQing 1
1School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, 2Acupuncture and Moxibustion Department, Beijing Hospital of Traditional Chinese Medicine affiliated with Capital Medical University, 3The Third Affiliated Hospital of Beijing University of Chinese Medicine

In this protocol, a knee osteoarthritis model was prepared using the modified Videman method, and the operation procedures and precautions of acupotomy are detailed. The effectiveness of acupotomy has been demonstrated by testing the mechanical properties of quadriceps femoris and tendon and the mechanical and morphological properties of cartilage.

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Neuroscience

Characteristics of Pain Changes in Rats with Nerve Injury Within 24 hours After One-Time Tuina Intervention
Zhenjie Yang 1, Sachula Wu 1, Tianyuan Yu 1, Jinping Chen 1, Runlong Zhang 1, Hourong Wang 2, Yingqi Zhang 1
1School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, 2Beijing Hospital

We present a protocol using the tuina manipulation simulator to perform the "Three-Manipulation and Three-Acupoint" tuina therapy for minor chronic constriction injury rats and evaluate the effective analgesic time points of tuina within 24 h by testing pain changes through behavioral analysis and changes in inflammatory factor expression using enzyme-linked immunosorbent assay.

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Medicine

Three-Dimensional Reconstruction for the Whole Lung with Early Multiple Pulmonary Nodules
Junyi Shi 1, Fangliang Xing 2, Yang Liu 3, Tengxiao Liang 4
1Beijing University of Chinese Medicine, 2Beijing Intelligent Entropy Science & Technology Co Ltd., 3Center for Integrated Chinese and Western Medicine, Beijing You An Hospital, Capital Medical University, 4Fever Outpatient Clinic, Dongzhimen Hospital of Beijing University of Chinese Medicine

This study introduces a three-dimensional (3D) reconstruction method for the entire lung in patients with early multiple pulmonary nodules. It offers a comprehensive visualization of nodule distribution and their interplay with lung tissue, simplifying the assessment of diagnosis and prognosis for these patients.

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A Rat Model of Compound Acne
Lin Chen *1,2, Qi Jin *1,2, Tian Xia *2, Xin Zhang 2, Ruiyi Cai 1, Xinyi Wu 2, Yue-Han Song 2, Guang-Zhong Zhang 1
1Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, 2School of Traditional Chinese Medicine, Beijing University of Chinese Medicine

This protocol outlines the procedure for inducing acne inflammation in rat skin with oleic acid and Cutibacterium acnes.

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Medicine

A 3D Quantification Technique for Liver Fat Fraction Distribution Analysis Using Dixon Magnetic Resonance Imaging
Fan Zhao 1, Genming Zhang 2, Zhongjian Tan 3, Tengxiao Liang 4, Fangliang Xing 5
1Department of Infectious Diseases, Dongzhimen Hospital, Beijing University of Chinese Medicine, 2The First Clinical College, Beijing University of Chinese Medicine, 3Department of Radiology, Dongzhimen Hospital, Beijing University of Chinese Medicine, 4Fever Clinics, Dongzhimen Hospital, Beijing University of Chinese Medicine, 5Beijing Intelligent Entropy Science & Technology Co Ltd.

This study introduces a unique 3D quantification method for liver fat fraction (LFF) distribution using Dixon Magnetic Resonance Imaging (Dixon MRI). LFF maps, derived from in-phase and water-phase images, are integrated with 3D liver contours to differentiate LFF patterns between normal and steatotic livers, enabling precise assessment of liver fat content.

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Medicine

The Effect of Anti-Fatigue Decoction on the Behaviors and Serological Indicators in a Central Fatigue Rat Model
Jie Li 1, Yi Liu 1, Ru-ting Li 1, Yan Liu 1, Feng Li 1
1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine

Here, we introduce a protocol to assess the effects of anti-fatigue decoction (AFD) on central fatigue in rats modeled by the modified multiple platform method (MMPM) by monitoring both their behavioral responses and serological markers.

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Medicine

Synchronous Triplanar Reconstruction Integrated with Color Doppler Mapping for Precise and Rapid Localization of Thyroid Lesions
Zhi Chen 1, Zhiguo Ding 1,2, Rui Hu 1, Tengxiao Liang 3, Fangliang Xing 4, Shuo Qi 1,2
1Department of Thyroid, Sunsimiao Hospital, Beijing University of Chinese Medicine, 2Department of Thyroid, Dongzhimen Hospital, Beijing University of Chinese Medicine, 3Fever Clinics, Dongzhimen Hospital, Beijing University of Chinese Medicine, 4Beijing Intelligent Entropy Science & Technology Co Ltd.

Here we present a 5D ultrasound technique combining multi-planar 3D reconstruction and color Doppler fusion, which enables synchronous visualization of thyroid structural and functional information. By minimizing blind spots, this method allows rapid, precise localization of lesions to improve diagnostic accuracy, especially benefiting novice practitioners.

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Medicine

Isolation of Umbilical Cord-Derived Mesenchymal Stem Cells with High Yields and Low Damage
Mingyuan Xu 1,2, Jiasen Xu 2, Danling Cheng 3,4, Xiaoni Chen 2, Shan Lin 2, Xinlei Si 2, Fangfang Guo 3,4, Di Wu 2, Fenfang Wu 2
1Beijing University of Chinese Medicine, 2Department of Central Laboratory, Shenzhen Hospital, Beijing University of Chinese Medicine, 3Obstetrical Department, Longgang District Maternity & Child Healthcare Hospital of Shenzhen City, 4Longgang Maternity and Child Institute of Shantou University, Medical College

This study presents a unique blunt dissection procedure to preserve the integrity of Wharton's jelly (WJ), resulting in less damaged WJ and a greater quantity and viability of the harvested mesenchymal stem cells (MSCs). The method demonstrates superior yield and proliferative ability compared to conventional sharp dissection methods.

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Medicine

Construction and Application of Cerebral Functional Region-Based Cerebral Blood Flow Atlas Using Magnetic Resonance Imaging-Arterial Spin Labeling
Zhongjian Tan 1, Fangliang Xing 2, Liping Zhang 1
1Department of Radiology, Dongzhimen Hospital, Beijing University of Chinese Medicine, 2Beijing Intelligent Entropy Science & Technology Co Ltd.

This study integrated magnetic resonance imaging- arterial spin labeling images to derive cerebral blood flow (CBF) atlas for cerebral functional regions. Comparing typical healthy and chronic cerebral ischemia CBF atlases revealed significant differences in regional CBF distributions, enabling rapid, noninvasive assessments of functional CBF to assist in diagnosis and evaluate therapeutics.

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Medicine

Tension-Free Weight-Bearing Model of Steroid-Induced Osteonecrosis of Femoral Head in Rats
Yue Zhang 1, Jiancheng Tang 1, Zhengrong Yue 1, Wanshou Guo 2,3, Weiguo Wang 2,3
1Graduate School, Beijing University of Chinese Medicine, 2Beijing Key Lab of Immune-Mediated Inflammatory Diseases, China-Japan Friendship Hospital, 3Department of Orthopedic and Joint Surgery, China-Japan Friendship Hospital

The protocol shows building a simple and cost-effective rat weight-bearing training model for steroid-induced osteonecrosis of the femoral head using elastic therapeutic tape.

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Medicine

Primary Sjogren's Syndrome Associated with Lung Adenocarcinoma: Probing the Potential Common Pathogenic Mechanisms and Experimental Verification
Ying Li 1, Xue-lei Chu 2, Peng Xue 2, Peng Wang 3, Ting-ting Zhou 4, Shi-jie Zhu 2
1Graduate School, Beijing University of Chinese Medicine, 2Oncology Department, Wangjing Hospital, China Academy of Chinese Medical Sciences, 3The Second Department of Healthcare, China-Japan Friendship Hospital, 4Rheumatology Department, Shangqiu Hospital of Traditional Chinese Medicine

This manuscript describes the operational procedures and precautions to probe the potential common pathogenic mechanisms linking primary Sjogren's syndrome and lung adenocarcinoma through bioinformatics analysis and experimental verification.

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