Generation of Enterobacter sp. YSU Auxotrophs Using Transposon MutagenesisJonathan James Caguiat 1
1Department of Biological Sciences, Center for Applied Chemical Biology, Youngstown State University
Enterobacter sp. YSU grows in glucose minimal salts medium. Auxotrophs are generated by transforming it with a transposome which randomly inserts itself into the host genome. Mutants are found by replica plating from complex medium to minimal medium. Interrupted genes are identified by gene rescue and sequencing.
