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University of Texas MD Anderson Cancer Center

9 ARTICLES PUBLISHED IN JoVE

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Medicine

Working with Human Tissues for Translational Cancer Research
Alexandre Reuben *1, Vancheswaran Gopalakrishnan *1, Heidi E. Wagner 3, Christine N. Spencer 2, Jacob Austin-Breneman 1, Hong Jiang 1, Zachary A. Cooper 1, Jennifer A. Wargo 1
1Department of Surgical Oncology, University of Texas MD Anderson Cancer Center, 2Department of Genomic Medicine, University of Texas MD Anderson Cancer Center, 3Department of Pathology and Institutional Tissue Bank, University of Texas MD Anderson Cancer Center

Translational cancer research is dependent on extraction of human tissues. Much work has gone into optimizing extraction methods for ex vivo analysis. Here, we describe tissue processing methods allowing for maximal data output from limited samples.

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Developmental Biology

Technique to Target Microinjection to the Developing Xenopus Kidney
Bridget D. DeLay 1, Vanja Krneta-Stankic 1,2, Rachel K. Miller 1,2,3,4
1Department of Pediatrics, Pediatric Research Center, University of Texas McGovern Medical School, 2Program in Genes & Development, University of Texas Graduate School of Biomedical Sciences, 3Program in Cell & Regulatory Biology, University of Texas Graduate School of Biomedical Sciences, 4Department of Genetics, University of Texas MD Anderson Cancer Center

Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.

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Biology

En Face Preparation of Mouse Blood Vessels
Kyung Ae Ko 1, Keigi Fujiwara 1, Sunil Krishnan 2, Jun-ichi Abe 1
1Department of Cardiology, Division of Internal Medicine, University of Texas MD Anderson Cancer Center, 2Department of Radiation Oncology, University of Texas MD Anderson Cancer Center

A procedure for making en face preparations of the mouse carotid artery and aorta is described. Such preparations, when immunofluorescently stained with specific antibodies, enable us to study localization of proteins and identification of cell types within the entire vascular wall by confocal microscopy.

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Immunology and Infection

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
Cara L. Haymaker *1, Yared Hailemichael *1, Yi Yang 2,3, Roza Nurieva 2
1Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center, 2Department of Immunology, University of Texas MD Anderson Cancer Center, 3Department of Radiation Oncology, The Second Hospital of Jilin University

The goal of this protocol is to allow for detection of in vivo antigen-specific killing of a target cell in a murine model.

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Cancer Research

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
Magdalena Delgado *1, Anisha Kothari *1, Walter N. Hittelman 2, Timothy C. Chambers 1
1Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 2Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center

This protocol describes the use of centrifugal elutriation to separate primary acute lymphoblastic leukemia cells into different cell cycle phases.

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Cancer Research

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
Christopher Terranova 1, Ming Tang 1, Elias Orouji 1, Mayinuer Maitituoheti 1, Ayush Raman 1, Samirkumar Amin 2, Zhiyi Liu 1, Kunal Rai 1
1Department of Genomic Medicine, University of Texas MD Anderson Cancer Center, 2The Jackson Laboratory for Genomic Medicine

Here, we describe an optimized high-throughput ChIP-sequencing protocol and computational analyses pipeline for the determination of genome-wide chromatin state patterns from frozen tumor tissues and cell lines.

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Medicine

Radiation Planning Assistant - A Streamlined, Fully Automated Radiotherapy Treatment Planning System
Laurence E. Court 1, Kelly Kisling 1, Rachel McCarroll 1, Lifei Zhang 1, Jinzhong Yang 1, Hannah Simonds 2, Monique du Toit 2, Chris Trauernicht 2, Hester Burger 3, Jeannette Parkes 3, Mike Mejia 4, Maureen Bojador 4, Peter Balter 1, Daniela Branco 1, Angela Steinmann 1, Garrett Baltz 1, Skylar Gay 1, Brian Anderson 1, Carlos Cardenas 1, Anuja Jhingran 5, Simona Shaitelman 5, Oliver Bogler 6, Kathleen Schmeller 7, David Followill 1, Rebecca Howell 1, Christopher Nelson 1, Christine Peterson 8, Beth Beadle 5,9
1Department of Radiation Physics, University of Texas MD Anderson Cancer Center, 2Department of Radiation Oncology, Stellenbosch University and Tygerberg Hospital, 3Departments of Radiation Oncology and Medical Physics, Groote Schuur Hospital and University of Cape Town, 4Department of Radiation Oncology, University of Santo Tomas Hospital, Benavides Cancer Institute, 5Department of Radiation Oncology, University of Texas MD Anderson Cancer Center, 6Academic Affairs, University of Texas MD Anderson Cancer Center, 7Department of Gynecological Oncology and Reproductive Medicine, University of Texas MD Anderson Cancer Center, 8Department of Biostatistics, University of Texas MD Anderson Cancer Center, 9Department of Radiation Oncology, Stanford University

Radiation therapy is a highly complex cancer treatment that requires multiple specialists to create a treatment plan and provide quality assurance (QA) prior to delivery to a patient. This protocol describes the use of a fully automated system, the Radiation Planning Assistant (RPA), to create high-quality radiation treatment plans.

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Cancer Research

Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
Tamara M. Griffiths 1, Carlos Ramos 1, Cara L. Haymaker 1
1Department of Translational Molecular Pathology, University of Texas MD Anderson Cancer Center

The goal of this method paper is to demonstrate a robust and reproducible methodology for the enrichment, generation, and expansion of primary tumor cell lines from surgically resected pleural mesothelioma.

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Neuroscience

Mechanical Conflict-Avoidance Assay to Measure Pain Behavior in Mice
Caitlyn M. Gaffney 1, Gabriella Muwanga 2, Huaishuang Shen 2, Vivianne L. Tawfik 2, Andrew J. Shepherd 2
1Laboratories of Neuroimmunology, Department of Symptom Research, and the MD Anderson Pain Research Consortium, University of Texas MD Anderson Cancer Center, 2Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine

The mechanical conflict-avoidance assay is used as a non-reflexive readout of pain sensitivity in mice which can be used to better understand affective-motivational responses in a variety of mouse pain models.

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