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Technique to Target Microinjection to the Developing Xenopus Kidney

DOI :

10.3791/53799-v

May 3rd, 2016

May 3rd, 2016

10,878 Views

1Department of Pediatrics, Pediatric Research Center, University of Texas McGovern Medical School, 2Program in Genes & Development, University of Texas Graduate School of Biomedical Sciences, 3Program in Cell & Regulatory Biology, University of Texas Graduate School of Biomedical Sciences, 4Department of Genetics, University of Texas MD Anderson Cancer Center

Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.

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Keywords Microinjection

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